| Author | Pinho, Rosa Teixeira de | |
| Author | Silva, Wellington Seguins da | |
| Author | Côrtes, Luzia Monteiro de Castro | |
| Author | Souza, Periela da Silva Vasconcelos | |
| Author | Soares, Renata Oliveira de Araujo | |
| Author | Alves, Carlos Roberto | |
| Access date | 2015-05-04T16:34:36Z | |
| Available date | 2015-05-04T16:34:36Z | |
| Document date | 2014 | |
| Citation | PINHO, Rosa Teixeira de et al. Production of MMP-9 and inflammatory cytokines by Trypanosoma cruzi-infected macrophages. Experimental Parasitology, v.147, p.72–80, 2014. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10182 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Citocinas | pt_BR |
| Title | Production of MMP-9 and inflammatory cytokines by Trypanosoma cruzi-infected macrophages | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2014.09.003 | pt_BR |
| Abstract | Matrix metalloproteinases (MMPs) constitute a large family of Zn2+ and Ca2+ dependent endopeptidases
implicated in tissue remodeling and chronic inflammation. MMPs also play key roles in the activation
of growth factors, chemokines and cytokines produced by many cell types, including lymphocytes, granulocytes,
and, in particular, activated macrophages. Their synthesis and secretion appear to be important
in a number of physiological processes, including the inflammatory process. Here, we investigated the
interaction between human and mouse macrophages with T. cruzi Colombian and Y strains to characterize
MMP-9 and cytokine production in this system. Supernatants and total extract of T. cruzi infected
human and mouse macrophages were obtained and used to assess MMP-9 profile and inflammatory
cytokines. The presence of metalloproteinase activity was determined by zymography, enzyme-linked
immunosorbent assay and immunoblotting assays. The effect of cytokines on MMP-9 production in human
macrophages was verified by previous incubation of cytokines on these cells in culture, and analyzed
by zymography. We detected an increase in MMP-9 production in the culture supernatants of T. cruzi
infected human and mouse macrophages. The addition of IL-1β or TNF-α to human macrophage cultures
increased MMP-9 production. In contrast, MMP-9 production was down-modulated when human
macrophage cultures were treated with IFN-γ or IL-4 before infection. Human macrophages infected with
T. cruzi Y or Colombian strains produced increased levels of MMP-9, which was related to the production
of cytokines such as IL-1β, TNF-α and IL-6. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil / Fundação Ataulpho de Paiva. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Matrix metalloproteinase-9 | pt_BR |
| Subject | Y strain | pt_BR |
| Subject | Colombian strain | pt_BR |
| Subject | Human macrophages | pt_BR |
| Subject | Mouse macrophages | pt_BR |
| Subject | THP-1 macrophages | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject in Spanish | Trypanosoma cruzi | pt_BR |
| Subject in Spanish | Citocinas | pt_BR |
| Subject in Spanish | Ciências Biológicas | pt_BR |
| DeCS | Citocinas | pt_BR |
