| Author | Barbieri, Raquel Rodrigues | |
| Author | Sales, Anna Maria | |
| Author | Illarramendi Rojas, Ximena | |
| Author | Moraes, Milton Ozório | |
| Author | Nery, José Augusto da Costa | |
| Author | Moreira, Suelen Justo Maria | |
| Author | Sarno, Euzenir Nunes | |
| Author | Machado, Alice de Miranda | |
| Author | Bozza, Fernando A. | |
| Access date | 2015-05-04T16:52:49Z | |
| Available date | 2015-05-04T16:52:49Z | |
| Document date | 2014 | |
| Citation | BARBIERI, Raquel Rodrigues et al. Diagnostic challenges of single plaque-like lesion paucibacillary leprosy. Memórias do Instituto Oswaldo Cruz, v. 109, n. 7, p. 944-947, Nov. 2014. | |
| ISSN | 0074-0276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10188 | |
| Description | Produção científica do Laboratório de Hanseníase. | pt_BR |
| Language | eng | en_US |
| Publisher | Fiocruz/IOC | |
| Rights | open access | |
| Title | Diagnostic challenges of single plaque-like lesion paucibacillary leprosy | en_US |
| Type | Article | |
| DOI | 10.1590/0074-0276140212 | |
| Abstract | The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Medicina Intensiva. Rio de Janeiro, RJ, Brasil. | |
| Subject | Paucibacillary leprosy | en_US |
| Subject | Diagnosis | en_US |
| Subject | Quantitative polymerase chain reaction | en_US |
| DeCS | Hanseníase Paucibacilar | pt_BR |
| DeCS | Mycobacterium leprae | pt_BR |
| DeCS | Diagnóstico | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| e-ISSN | 1678-8060 | |
