| Author | Vieira, Yasmine Rangel | |
| Author | Santos, Debora Regina Lopes dos | |
| Author | Portilho, Moyra Machado | |
| Author | Velloso, Carlos Eduardo Pereira | |
| Author | Arissawa, Marcia | |
| Author | Villar, Livia Melo | |
| Author | Pinto, Marcelo Alves | |
| Author | Paula, Vanessa Salete de | |
| Access date | 2015-05-08T14:08:47Z | |
| Available date | 2015-05-08T14:08:47Z | |
| Document date | 2014 | |
| Citation | VIEIRA, Yasmine Rangel et al. Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs. BMC Microbiology, v.14, n.315, 6p, 2014. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10271 | |
| Language | eng | pt_BR |
| Publisher | Biomed Central | pt_BR |
| Rights | open access | |
| Title | Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs | pt_BR |
| Type | Article | |
| DOI | 10.1186/s12866-014-0315-2 | pt_BR |
| Abstract | Background: Preliminary studies showed the prevalence of a virus similar to human hepatitis B virus (HBV-like) in
swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil.
In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in
certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential.
Results: Bile and liver samples from swine were positive for partial genome amplification (ORF S and ORF C), direct
sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification
of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the
presence of HBsAg antigen in liver tissue sections.
Conclusions: So far our data suggest that commercial swine house an HBV-like virus and this relevant finding should
be considered in studies on the origin and viral evolution. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Departamento de Mircobiologia Veterinária e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hepadnavirus | pt_BR |
| Subject | Commercial swine | pt_BR |
| Subject | Brazil | pt_BR |
| Subject | Bile | pt_BR |
| Subject | Liver | pt_BR |
| Subject | Genotyping | pt_BR |
| Subject | Immunofluorescence | pt_BR |
| DeCS | Imunofluorescência | pt_BR |
| DeCS | Técnicas de Genotipagem | pt_BR |
| DeCS | Bile | pt_BR |
| DeCS | Fígado | pt_BR |
