| Author | Wassaf, Maribel G. Martinez | |
| Author | Pisano, María B | |
| Author | Barril, Patricia A | |
| Author | Elbarcha, Osvaldo C | |
| Author | Pinto, Marcelo A | |
| Author | Oliveira, Jaqueline Mendes de | |
| Author | DiGiusto, Pablo | |
| Author | Nates, Silvia V | |
| Author | Ré, Viviana E | |
| Access date | 2015-05-08T14:08:47Z | |
| Available date | 2015-05-08T14:08:47Z | |
| Document date | 2014 | |
| Citation | WASSAF, Maribel G. Martínez et al. First detection of hepatitis E virus in Central Argentina: Environmental and serological survey. Journal of Clinical Virology, v.61,n.3, p.313-472, 2014. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10272 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | First detection of hepatitis E virus in Central Argentina: Environmental and serological survey | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jcv.2014.08.016 | pt_BR |
| Abstract | Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide,
transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not
routinely performed. Besides, in Argentina’s central area epidemiological and molecular characteristics
of HEV are still unknown.
Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples
and by serological survey in healthy adult population of Córdoba city, Argentina.
Study design: Environmental surveillance was conducted in river and sewage samples collected between
2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial
regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010.
Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide
sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence
of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI
1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical
relation was found between anti-HEV and socioeconomic level.
Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates
in central Argentina. Contamination of water with HEV could represent a route of transmission for local
populations, which have a high number of susceptible individuals. This fact alerts local health care systems
in order to include detection of HEV in the diagnostic algorithm of viral hepatitis. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina / LACE Laboratories. Department of Virology and Molecular Biology. Córdoba, Argentina. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina / National University of Córdoba. School of Medical Sciences. Virology Institute Dr. J. M. Vanella. Córdoba, Argentina. | pt_BR |
| Affilliation | National University of Córdoba. School of Medical Sciences. Virology Institute Dr. J. M. Vanella. Córdoba, Argentina. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina / LACE Laboratories. Department of Virology and Molecular Biology. Córdoba, Argentina. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina. | pt_BR |
| Affilliation | Catholic University of Córdoba. School of Chemical Sciences. Department of Virology. Córdoba, Argentina / National University of Córdoba. School of Medical Sciences. Virology Institute Dr. J. M. Vanella. Córdoba, Argentina. | pt_BR |
| Subject | Hepatitis E virus | pt_BR |
| Subject | Environmental surveillance | pt_BR |
| Subject | Seroprevalence | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| Subject | Subtyping | pt_BR |
| Subject | Argentina | pt_BR |
| DeCS | Epidemiologia Molecular | pt_BR |
| DeCS | Estudos Soroepidemiológicos | pt_BR |
| DeCS | Imunofenotipagem | pt_BR |
| DeCS | Vírus da Hepatite E | pt_BR |
