| Author | Soares, Juliana Baptista Rocha Corrêa | |
| Author | Monteiro, Clarissa Menezes Maya | |
| Author | Cunha, Paula do Rego Barros Bittencourt | |
| Author | Atella, Georgia Correa | |
| Author | Lara, Flávio Alves | |
| Author | Avila, Joana da Costa Pinto d´ | |
| Author | Menezes, Diego Silva | |
| Author | Santos, Marcos André Vannier dos | |
| Author | Oliveira, Pedro Lagerblad de | |
| Author | Egan, Timothy J | |
| Author | Oliveira, Marcus Fernandes de | |
| Access date | 2015-05-20T18:26:38Z | |
| Available date | 2015-05-20T18:26:38Z | |
| Document date | 2007 | |
| Citation | SOARES, J. B. R.C. Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni. FEBS Letters,v. 581, n. 9, p. 1742-1750, 2007. | pt_BR |
| ISSN | 0014-5793 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10444 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.febslet.2007.03.054 | |
| Abstract | Hemozoin (Hz) is a heme crystal produced upon
hemoglobin digestion as the main mechanism of heme disposal
in several hematophagous organisms. Here, we show that, in
the helminth Schistosoma mansoni, Hz formation occurs in
extracellular lipid droplets (LDs). Transmission electron microscopy
of adult worms revealed the presence of numerous electronlucent
round structures similar to LDs in gut lumen, where
multicrystalline Hz assemblies were found associated to their
surfaces. Female regurgitates promoted Hz formation in vitro
in reactions partially inhibited by boiling. Fractionation of regurgitates
showed that Hz crystallization activity was essentially
concentrated on lower density fractions, which have small
amounts of pre-formed Hz crystals, suggesting that hydrophilic–
hydrophobic interfaces, and not Hz itself, play a key catalytic
role in Hz formation in S. mansoni. Thus, these data
demonstrate that LDs present in the gut lumen of S. mansoni
support Hz formation possibly by allowing association of heme
to the lipid–water interface of these structures | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacteriose. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biomorfologia Parasitária. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | University of Cape Town. Department of Chemistry. Cape Tow, Sudáfrica | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Hemozoin | pt_BR |
| Subject | Heme | pt_BR |
| Subject | Lipid droplets | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| DeCS | Hemoproteínas | pt_BR |
| DeCS | Lípidos | pt_BR |
| DeCS | Schistosoma mansoni | pt_BR |
| DeCS | Hemozoin | pt_BR |
