| Author | Marinho, Cintia Ferreira | |
| Author | Azeredo, Elzinandes Leal | |
| Author | Carvalho, Amanda Torrentes | |
| Author | Santos, Alessandro Marins dos | |
| Author | Kubelka, Claire Fernandes | |
| Author | Souza, Luiz José de | |
| Author | Cunha, Rivaldo Venâncio | |
| Author | Pinto, Luiza Maria de Oliveira | |
| Access date | 2015-05-27T13:39:45Z | |
| Available date | 2015-05-27T13:39:45Z | |
| Document date | 2014 | |
| Citation | MARINHO, Cintia Ferreira et al. Down-Regulation of Complement Receptors on the Surface of Host Monocyte Even as In Vitro Complement Pathway Blocking Interferes in Dengue Infection. Plos One, v.9, n.7, 14p, 2014. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10529 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dengue | pt_BR |
| Title | Down-Regulation of Complement Receptors on the Surface of Host Monocyte Even as In Vitro Complement Pathway Blocking Interferes in Dengue Infection | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone | pt_BR |
| Abstract | In dengue virus (DENV) infection, complement system (CS) activation appears to have protective and pathogenic effects. In
severe dengue fever (DF), the levels of DENV non-structural-1 protein and of the products of complement activation,
including C3a, C5a and SC5b-9, are higher before vascular leakage occurs, supporting the hypothesis that complement
activation contributes to unfavourable outcomes. The clinical manifestations of DF range from asymptomatic to severe and
even fatal. Here, we aimed to characterise CS by their receptors or activation product, in vivo in DF patients and in vitro by
DENV-2 stimulation on monocytes. In comparison with healthy controls, DF patients showed lower expression of CR3
(CD11b), CR4 (CD11c) and, CD59 on monocytes. The DF patients who were high producers of SC5b-9 were also those that
showed more pronounced bleeding or vascular leakage. Those findings encouraged us to investigate the role of CS in vitro,
using monocytes isolated from healthy subjects. Prior blocking with CR3 alone (CD11b) or CR3 (CD11b/CD18) reduced viral
infection, as quantified by the levels of intracellular viral antigen expression and soluble DENV non-structural viral protein.
However, we found that CR3 alone (CD11b) or CR3 (CD11b/CD18) blocking did not influence major histocompatibility
complex presentation neither active caspase-1 on monocytes, thus probably ruling out inflammasome-related mechanisms.
Although it did impair the secretion of tumour necrosis factor alpha and interferon alpha. Our data provide strategies of
blocking CR3 (CD11b) pathways could have implications for the treatment of viral infection by antiviral-related mechanisms. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centro de Referência para Dengue. Campos de Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centro de Referência para Dengue. Campos de Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue | pt_BR |
| Subject | Dengue virus (DENV) infection | pt_BR |
| Subject in Spanish | Dengue | pt_BR |
