| Author | Santos, Théo de Araújo | |
| Author | Prates, Deboraci Brito | |
| Author | Costa, Jaqueline França | |
| Author | Luz, Nívea Farias | |
| Author | Andrade, Bruno de Bezerril | |
| Author | Miranda, José Carlos | |
| Author | Brodskyn, Claudia Ida | |
| Author | Barral, Aldina Maria Prado | |
| Author | Bozza, Patrícia T. | |
| Author | Borges, Valeria de Matos | |
| Access date | 2015-05-27T13:39:48Z | |
| Available date | 2015-05-27T13:39:48Z | |
| Document date | 2014 | |
| Citation | SANTOS, Théo Araújo et al. Prostaglandin E2/Leukotriene B4 balance induced by Lutzomyia longipalpis saliva favors Leishmania infantum infection. Parasites & Vectors, v.7:601, 8p, 2014. | pt_BR |
| ISSN | 1756-3305 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10541 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Title | Prostaglandin E2/Leukotriene B4 balance induced by Lutzomyia longipalpis saliva favors Leishmania infantum infection | pt_BR |
| Type | Article | |
| DOI | 10.1186/s13071-014-0601-8 | pt_BR |
| Abstract | Background
Eicosanoids and sand fly saliva have a critical role in the Leishmania infection. Here, we evaluated the effect of Lutzomyia longipalpis salivary gland sonicate (SGS) on neutrophil and monocyte recruitment and activation of eicosanoid production in a murine model of inflammation.
Methods
C57BL/6 mice were inoculated intraperitonealy with Lutzomyia longipalpis SGS or Leishmania infantum or both, followed by analyses of cell recruitment, parasite load and eicosanoid production.
Results
Intraperitoneal injection of Lutzomyia longipalpis SGS together with Leishmania infantum induced an early increased parasite viability in monocytes and neutrophils. L. longipalpis SGS increased prostaglandin E2 (PGE2), but reduced leukotriene B4 (LTB4) production ex vivo in peritoneal leukocytes. In addition, the pharmacological inhibition of cyclooxygenase 2 (COX-2) with NS-398 decreased parasite viability inside macrophages during Leishmania infection in the presence of L. longipalpis SGS arguing that PGE2 production is associated with diminished parasite killing.
Conclusions
These findings indicate that L. longipalpis SGS is a critical factor driving immune evasion of Leishmania through modulation of PGE2/LTB4 axis, which may represent an important mechanism on establishment of the infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia Ocidental. Centro de Ciências Biológicas e da Saúde. Barreiras, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Section of Immunobiology. Bethesda, MD, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunobiologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunobiologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Muniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunobiologia. São Paulo, SP, Brasil. | pt_BR |
| Subject | Lutzomyia longipalpis | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | Saliva | pt_BR |
| Subject | Prostaglandina E2 | pt_BR |
| Subject | Leukotriene B4 | pt_BR |
| DeCS | Leishmania infantum | pt_BR |
| DeCS | Leucotrieno B4 | pt_BR |
| DeCS | Saliva | pt_BR |
