| Author | Ribeiro Filho, Jaime | |
| Author | Leite, Fagner Carvalho | |
| Author | Costa, Hermann Ferreira | |
| Author | Calheiros, Andrea Surrage | |
| Author | Torres, Rafael Carvalho | |
| Author | Azevedo, Carolina Trindade de | |
| Author | Martins, Marco Aurélio | |
| Author | Dias, Celidarque da Silva | |
| Author | Bozza, Patricia Torres | |
| Author | Piuvezam, Márcia Regina | |
| Access date | 2015-05-27T13:39:52Z | |
| Available date | 2015-05-27T13:39:52Z | |
| Document date | 2014 | pt_BR |
| Citation | RIBEIRO FILHO, Jaime et al. Curine inhibits mast cell-dependent responses in mice. Journal of Ethnopharmacology, v.155, n.2, p.1118–1124, 2014. | pt_BR |
| ISSN | 0378-8741 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10553 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Curine inhibits mast cell-dependent responses in mice | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.jep.2014.06.041 | pt_BR |
| Abstract | Ethnopharmacological relevance
Curine is a bisbenzylisoquinoline alkaloid and the major constituent isolated from Chondrodendron platyphyllum, a plant that is used to treat inflammatory diseases in Brazilian folk medicine. This study investigates the effectiveness of curine on mast cell-dependent responses in mice.
Materials and methods
To induce mast cell-dependent responses, Swiss mice were subcutaneously sensitized with ovalbumin (OVA—12 μg/mouse) and Al(OH)3 in a 0.9% NaCl solution. Fifteen days later, the animals were challenged with OVA through different pathways. Alternatively, the animals were injected with compound 48/80 or histamine, and several parameters, including anaphylaxis, itching, edema and inflammatory mediator production, were analyzed. Promethazine, cromoglycate, and verapamil were used as control drugs, and all of the treatments were performed 1 h before the challenges.
Results
Curine pre-treatment significantly inhibited the scratching behavior and the paw edema induced by either compound 48/80 or OVA, and this protective effect was comparable in magnitude with those associated with treatment with either cromoglycate or verapamil. In contrast, curine was a weak inhibitor of histamine-induced paw edema, which was completely inhibited by promethazine. Curine and verapamil significantly inhibited pleural protein extravasations and prostaglandin D2 (PGD2) and cysteinyl leukotrienes (CysLTs) production following allergen-induced pleurisy. Furthermore, like verapamil, curine inhibited the anaphylactic shock caused by either compound 48/80 or an allergen. In in vitro settings, these treatments also inhibited degranulation as well as PGD2 and CysLT production through IgE-dependent activation of the mast cell lineage RBL-2H3.
Conclusion
Curine significantly inhibited immediate allergic reactions through mechanisms more related to mast cell stabilization and activation inhibition than interference with the pro-inflammatory effects of mast cell products. These findings are in line with the hypothesis that the alkaloid curine may be beneficial for the treatment of allergic disorders. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. Laboratório de Fitoquímica. João Pessoa, PB, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil. | pt_BR |
| Subject | Curine | pt_BR |
| Subject | Chondrodentron platyphyllum | pt_BR |
| Subject | Anti-allergic | pt_BR |
| Subject | Mast cell | pt_BR |
| Subject | Lipid mediators | pt_BR |
