| Author | Falcão, Sarah de Athayde Couto | |
| Author | Weinkopff, Tiffany | |
| Author | Hurrell, Benjamin P | |
| Author | Celes, Fabiana Santana | |
| Author | Curvelo, Rebecca Pereira | |
| Author | Prates, Deboraci Brito | |
| Author | Barral, Aldina Maria Prado | |
| Author | Borges, Valeria de Matos | |
| Author | Cottier, Fabienne Tacchini | |
| Author | Oliveira, Camila Indiani de | |
| Access date | 2015-05-28T16:18:59Z | |
| Available date | 2015-05-28T16:18:59Z | |
| Document date | 2015 | |
| Citation | FALCÃO, S. A. C. et al. Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. PLoS Neglected Tropical Diseases, v. 9, n. 3, p. e0003601, 2015. | pt_BR |
| ISSN | 1935-2727 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10576 | |
| Language | por | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Neutrófilos | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Apoptose | pt_BR |
| Subject in Portuguese | Animais | pt_BR |
| Subject in Portuguese | Ratos | pt_BR |
| Subject in Portuguese | Medula óssea | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Title | Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0003601 | |
| Abstract | BACKGROUND: Neutrophils are the first line of defense against invading pathogens and are rapidly recruited to the sites of Leishmania inoculation. During Leishmania braziliensis infection, depletion of inflammatory cells significantly increases the parasite load whereas co-inoculation of neutrophils plus L. braziliensis had an opposite effect. Moreover, the co-culture of infected macrophages and neutrophils also induced parasite killing leading us to ask how neutrophils alone respond to an L. braziliensis exposure. Herein we focused on understanding the interaction between neutrophils and L. braziliensis, exploring cell activation and apoptotic fate. METHODS AND FINDINGS: Inoculation of serum-opsonized L. braziliensis promastigotes in mice induced neutrophil accumulation in vivo, peaking at 24 h. In vitro, exposure of thyoglycollate-elicited inflammatory or bone marrow neutrophils to L. braziliensis modulated the expression of surface molecules such as CD18 and CD62L, and induced the oxidative burst. Using mCherry-expressing L. braziliensis, we determined that such effects were mainly observed in infected and not in bystander cells. Neutrophil activation following contact with L. braziliensis was also confirmed by the release of TNF-α and neutrophil elastase. Lastly, neutrophils infected with L. braziliensis but not with L. major displayed markers of early apoptosis. CONCLUSIONS: We show that L. braziliensis induces neutrophil recruitment in vivo and that neutrophils exposed to the parasite in vitro respond through activation and release of inflammatory mediators. This outcome may impact on parasite elimination, particularly at the early stages of infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerland | pt_BR |
| Affilliation | University of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerland | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerland | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasil | pt_BR |
