Mostrar registro simples

AutorFalcão, Sarah de Athayde Couto
AutorWeinkopff, Tiffany
AutorHurrell, Benjamin P
AutorCeles, Fabiana Santana
AutorCurvelo, Rebecca Pereira
AutorPrates, Deboraci Brito
AutorBarral, Aldina Maria Prado
AutorBorges, Valeria de Matos
AutorCottier, Fabienne Tacchini
AutorOliveira, Camila Indiani de
Data de acesso2015-05-28T16:18:59Z
Data de disponibilização2015-05-28T16:18:59Z
Data do publicação2015
CitaçãoFALCÃO, S. A. C. et al. Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis. PLoS Neglected Tropical Diseases, v. 9, n. 3, p. e0003601, 2015.pt_BR
ISSN1935-2727
URIhttps://www.arca.fiocruz.br/handle/icict/10576
Idiomaporpt_BR
EditorPublic Library of Sciencept_BR
Direito Autoralopen accesspt_BR
Palavras-chaveNeutrófilospt_BR
Palavras-chaveLeishmaniapt_BR
Palavras-chaveLeishmania braziliensispt_BR
Palavras-chaveApoptosept_BR
Palavras-chaveAnimaispt_BR
Palavras-chaveRatospt_BR
Palavras-chaveMedula ósseapt_BR
Palavras-chaveInfecçãopt_BR
TítuloExposure to Leishmania braziliensis triggers neutrophil activation and apoptosis.pt_BR
Tipo do documentoArticlept_BR
DOI10.1371/journal.pntd.0003601
Resumo em InglêsBACKGROUND: Neutrophils are the first line of defense against invading pathogens and are rapidly recruited to the sites of Leishmania inoculation. During Leishmania braziliensis infection, depletion of inflammatory cells significantly increases the parasite load whereas co-inoculation of neutrophils plus L. braziliensis had an opposite effect. Moreover, the co-culture of infected macrophages and neutrophils also induced parasite killing leading us to ask how neutrophils alone respond to an L. braziliensis exposure. Herein we focused on understanding the interaction between neutrophils and L. braziliensis, exploring cell activation and apoptotic fate. METHODS AND FINDINGS: Inoculation of serum-opsonized L. braziliensis promastigotes in mice induced neutrophil accumulation in vivo, peaking at 24 h. In vitro, exposure of thyoglycollate-elicited inflammatory or bone marrow neutrophils to L. braziliensis modulated the expression of surface molecules such as CD18 and CD62L, and induced the oxidative burst. Using mCherry-expressing L. braziliensis, we determined that such effects were mainly observed in infected and not in bystander cells. Neutrophil activation following contact with L. braziliensis was also confirmed by the release of TNF-α and neutrophil elastase. Lastly, neutrophils infected with L. braziliensis but not with L. major displayed markers of early apoptosis. CONCLUSIONS: We show that L. braziliensis induces neutrophil recruitment in vivo and that neutrophils exposed to the parasite in vitro respond through activation and release of inflammatory mediators. This outcome may impact on parasite elimination, particularly at the early stages of infection.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaçãoUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerlandpt_BR
AfiliaçãoUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerlandpt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaçãoUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, Brasilpt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasilpt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasilpt_BR
AfiliaçãoUniversity of Lausanne. Department of Biochemistry and WHO-Immunology Research and Training Center. Epalinges, Switzerlandpt_BR
AfiliaçãoFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brasilpt_BR


Arquivos deste item

application/pdf
Nome:
Falcao SAC Exposure....pdf
Tamanho:
1.679Mb
Formato:
application/
Visualizar/Abrir

Este item aparece na(s) seguinte(s) coleção(s)

Mostrar registro simples