Author | Oliveira, Tatiane Teixeira | |
Author | Campos, Keina Maciele | |
Author | Lima, Ana Tereza Cerqueira | |
Author | Carneiro, Tamires Cana Brasil | |
Author | Velozo, Eudes da Silva | |
Author | Melo, Ingrid Christie Alexandrino Ribeiro | |
Author | Figueiredo, Eugênia Abrantes | |
Author | Oliveira, Eduardo de Jesus | |
Author | Vasconcelos, Dariz Flávia Silva Amorim de | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Neves, Neuza Maria Alcântara | |
Author | Figueiredo, Camila Alexandrina Viana | |
Access date | 2015-05-29T13:18:09Z | |
Available date | 2015-05-29T13:18:09Z | |
Document date | 2015 | |
Citation | OLIVEIRA, T. T. et al. Potential therapeutic effect of Allium cepa L. and quercetin in a murine model of Blomia tropicalis induced asthma. DARU Journal of Pharmaceutical Sciences, v.23, p. 18, 2015. | pt_BR |
ISSN | 1560-8115 | |
URI | https://www.arca.fiocruz.br/handle/icict/10582 | |
Language | eng | pt_BR |
Publisher | Tehran University of Medical Sciences | pt_BR |
Rights | open access | pt_BR |
Title | Potential therapeutic effect of Allium cepa L. and quercetin in a murine model of Blomia tropicalis induced asthma | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s40199-015-0098-5 | |
Abstract | Background: Asthma is an inflammatory condition characterized by airway hyperresponsiveness and chronic
inflammation. The resolution of inflammation is an essential process to treat this condition. In this study we
investigated the effect of Allium cepa L. extract (AcE) and quercetin (Qt) on cytokine and on smooth muscle
contraction in vitro and its therapeutic potential in a murine model of asthma.
Methods: AcE was obtained by maceration of Allium cepa L. and it was standardized in terms of quercetin
concentration using high performance liquid chromatography (HPLC). In vitro, using AcE 10, 100 or 1000 μg/ml or
Qt 3.5, 7.5, 15 μg/ml, we measured the concentration of cytokines in spleen cell culture supernatants, and the
ability to relax tracheal smooth muscle from A/J mice. In vivo, Blomia tropicalis (BT)-sensitized A/J mice were treated
with AcE 100, 1000 mg/kg or 30 mg/kg Qt. We measured cell influx in bronchoalveolar lavage (BAL), eosinophil
peroxidase (EPO) in lungs, serum levels of Bt-specific IgE, cytokines levels in BAL, and lung histology.
Results: We observed a reduction in the production of inflammatory cytokines, a relaxation of tracheal rings, and a
reduction in total number of cells in BAL and EPO in lungs by treatment with AcE or Qt.
Conclusion: AcE and Qt have potential as antiasthmatic drugs, as they possess both immunomodulatory and
bronchodilatory properties. | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Paraíba. Centro de Biotecnologia. João Pessoa, PB, Brasil | pt_BR |
Affilliation | Universidade Federal da Paraíba. Centro de Biotecnologia. João Pessoa, PB, Brasil | pt_BR |
Affilliation | Universidade Federal da Paraíba. Centro de Biotecnologia. João Pessoa, PB, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Subject | Natural product | pt_BR |
Subject | Asthma | pt_BR |
Subject | Blomia tropicalis | pt_BR |
Subject | Allium cepa L | pt_BR |
Subject | Quercetin | pt_BR |