| Author | Smaniotto, Salete | |
| Author | Schneider, Elke | |
| Author | Goudin, Nicolas | |
| Author | Bricard-Rignault, Rachel | |
| Author | Machavoine, François | |
| Author | Dardenne, Mireille | |
| Author | Dy, Michel | |
| Author | Savino, Wilson | |
| Access date | 2015-06-08T14:01:47Z | |
| Available date | 2015-06-08T14:01:47Z | |
| Document date | 2013 | |
| Citation | SMANIOTTO, Salete et al. Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility. Plos One, v.8, n.9, 8p, sept. 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10679 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Title | Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0070292 | pt_BR |
| Abstract | Basophils co-express FceRIa and CD49b, the a-2 chain of integrin-type receptor VLA-2 (a2b1), which recognizes type-1
collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone
marrow matrix remains unknown. Herein, we examined the expression of several receptors of this family by bone marrowderived
basophils sorted either ex-vivo or after culture with IL-3. Having established that both populations display CD49d,
CD49e and CD49f (a-4, a-5 and a-6 integrins subunits, respectively), we addressed receptor functions by measuring
migration, adhesion, proliferation and survival after interacting with matched natural ligands. Type I collagen, laminin and
fibronectin promoted basophil migration/adhesion, the former being the most effective. None of these ligands affected
basophil viability and expansion. Interactions between basophils and extracellular matrix are likely to play a role in situ, as
supported by confocal 3D cell imaging of femoral bone marrow sections, which revealed basophils exclusively in type-1
collagen-enriched niches that contained likewise laminin and fibronectin. This is the first evidence for a structure/function
relationship between basophils and extracellular matrix proteins inside the mouse bone marrow. | pt_BR |
| Affilliation | Universidade Federal de Alagoas. Instituto de Ciências Biológicas e da Saúde. Laboratório de Biologia Celular.. Maceió, AL, Brasil. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. Cell Imaging Platform. Paris, France. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France. | pt_BR |
| Affilliation | Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mouse Basophils | pt_BR |
| Subject | Bone Marrow | pt_BR |
| Subject | Extracellular Matrix | pt_BR |
| DeCS | Medula Òssea | pt_BR |
| DeCS | Basófilos | pt_BR |
| DeCS | Matriz Extracelular | pt_BR |
