| Author | Mesquita, Milene | |
| Author | Rodrigues, Natalia Fintelman | |
| Author | Sacramento, Carolina Q. | |
| Author | Abrantes, Juliana L. | |
| Author | Costa, Eduardo | |
| Author | Temerozo, Jairo R. | |
| Author | Siqueira, Marilda Agudo Mendonça Teixeira de | |
| Author | Bou-Habib, Dumith Chequer | |
| Author | Souza, Thiago Moreno L. | |
| Access date | 2015-06-08T14:01:48Z | |
| Available date | 2015-06-08T14:01:48Z | |
| Document date | 2014 | |
| Citation | MESQUITA, Milene et al. HIV-1 and Its gp120 Inhibits the Influenza A(H1N1)pdm09 Life Cycle in an IFITM3-Dependent Fashion. Plos One, v. 9, n. 6, p. 1-14, Jun. 2014. | pt_BR |
| ISSN | 1932-6203 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10683 | |
| Description | Thiago Moreno Lopes e Souza - Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Title | HIV-1 and Its gp120 Inhibits the Influenza A(H1N1)pdm09 Life Cycle in an IFITM3-Dependent Fashion | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0101056 | pt_BR |
| Abstract | HIV-1-infected patients co-infected with A(H1N1)pdm09 surprisingly presented benign clinical outcome. The knowledge that HIV-1 changes the host homeostatic equilibrium, which may favor the patient resistance to some co-pathogens, prompted us to investigate whether HIV-1 infection could influence A(H1N1)pdm09 life cycle in vitro. We show here that exposure of A(H1N1)pdm09-infected epithelial cells to HIV-1 viral particles or its gp120 enhanced by 25% the IFITM3 content, resulting in a decrease in influenza replication. This event was dependent on toll-like receptor 2 and 4. Moreover, knockdown of IFITM3 prevented HIV-1 ability to inhibit A(H1N1)pdm09 replication. HIV-1 infection also increased IFITM3 levels in human primary macrophages by almost 100%. Consequently, the arrival of influenza ribonucleoproteins (RNPs) to nucleus of macrophages was inhibited, as evaluated by different approaches. Reduction of influenza RNPs entry into the nucleus tolled A(H1N1)pdm09 life cycle in macrophages earlier than usual, limiting influenza’s ability to induce TNF-a. As judged by analysis of the influenza hemagglutin (HA) gene from in vitro experiments and from samples of HIV-1/ A(H1N1)pdm09 co-infected individuals, the HIV-1-induced reduction of influenza replication resulted in delayed viral evolution. Our results may provide insights on the mechanisms that may have attenuated the clinical course of Influenza in HIV-1/A(H1N1)pdm09 co-infected patients during the recent influenza form 2009/2010. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virus Respiratório e do Sarampo. WHO/NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | HIV-1 | pt_BR |
| Subject | Influenza A(H1N1)pdm09 | pt_BR |
| DeCS | HIV-1 | pt_BR |
