| Author | Amorim, M. R. | |
| Author | Vargas, F. R. | |
| Author | Llerena Junior, Juan Clinton | |
| Author | Pombo-de-Oliveira, M. S. | |
| Access date | 2015-06-09T16:41:43Z | |
| Available date | 2015-06-09T16:41:43Z | |
| Document date | 2007 | |
| Citation | AMORIM, M. R. et al. DNA extraction from fixed cytogenetic cell suspensions. Genetics and Molecular Research, Ribeirao Preto, v. 6, n. 3, p. 500-503, 2007. | pt_BR |
| ISSN | 1676-5680 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10749 | |
| Language | eng | pt_BR |
| Publisher | Fundação Norte-Rio-Grandense de Pesquisa e Cultura | pt_BR |
| Rights | open access | |
| Title | DNA extraction from fixed cytogenetic cell suspensions | pt_BR |
| Type | Article | pt_BR |
| Abstract | AbstRACt. We developed a procedure for DNA extraction from
small volumes of fixed cell suspensions previously prepared for con-ventional cytogenetic analysis. Good quality DNA was isolated with a
fast and simple protocol using DNAzol reagent. This provided suitable
DNA for various types of molecular analyses, including polymerase
chain reaction, restriction fragment length polymorphism, denaturing
high-performance liquid chromatography, and direct sequencing. This
technique provides sufficient material for such test, which are important
for diagnosis of neoplastic diseases in pediatric patients. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Genética. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Estado do Rio de Janeiro. Unidade de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Divisão de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | DNA Extraction | pt_BR |
| Subject | DNAzol | pt_BR |
| Subject | Cell Suspension | pt_BR |
| Subject | Cytogenetic | pt_BR |
| DeCS | DNA | pt_BR |
| DeCS | Citogenética | pt_BR |
