| Author | Lago, Barbara V. | |
| Author | Mello, Francisco C. | |
| Author | Ribas, Flavia S. | |
| Author | Valente, Fatima | |
| Author | Soares, Caroline C. | |
| Author | Niel, Christian | |
| Author | Gomes, Selma A. | |
| Access date | 2015-06-12T13:57:49Z | |
| Available date | 2015-06-12T13:57:49Z | |
| Document date | 2014 | pt_BR |
| Citation | LAGO, Barbara V. et al. Analysis of Complete Nucleotide Sequences of Angolan Hepatitis B Virus Isolates Reveals the Existence of a Separate Lineage within Genotype E. Plos One, v.9, n.3, 10p, mar. 2014. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10811 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Hepatite B | pt_BR |
| Subject in Portuguese | Angola | pt_BR |
| Subject in Portuguese | Genótipo E | pt_BR |
| Title | Analysis of Complete Nucleotide Sequences of Angolan Hepatitis B Virus Isolates Reveals the Existence of a Separate Lineage within Genotype E | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0092223 | pt_BR |
| Abstract | Hepatitis B virus genotype E (HBV/E) is highly prevalent in Western Africa. In this work, 30 HBV/E isolates from HBsAg
positive Angolans (staff and visitors of a private hospital in Luanda) were genetically characterized: 16 of them were
completely sequenced and the pre-S/S sequences of the remaining 14 were determined. A high proportion (12/30, 40%) of
subjects tested positive for both HBsAg and anti-HBs markers. Deduced amino acid sequences revealed the existence of
specific substitutions and deletions in the B- and T-cell epitopes of the surface antigen (pre-S1- and pre-S2 regions) of the
virus isolates derived from 8/12 individuals with concurrent HBsAg/anti-HBs. Phylogenetic analysis performed with 231 HBV/
E full-length sequences, including 16 from this study, showed that all isolates from Angola, Namibia and the Democratic
Republic of Congo (n = 28) clustered in a separate lineage, divergent from the HBV/E isolates from nine other African
countries, namely Cameroon, Central African Republic, Coˆ te d’Ivoire, Ghana, Guinea, Madagascar, Niger, Nigeria and Sudan,
with a Bayesian posterior probability of 1. Five specific mutations, namely small S protein T57I, polymerase Q177H, G245W
and M612L, and X protein V30L, were observed in 79-96% of the isolates of the separate lineage, compared to a frequency
of 0–12% among the other HBV/E African isolates. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Ministério da Saúde. Fundação Eduardo dos Santos. Luanda, Angola. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hepatitis B | pt_BR |
| Subject | Genotype E | pt_BR |
