| Author | Gómez, Mariela Martínez | |
| Author | Costa, Filipe Aníbal Carvalho | |
| Author | Volotão, Eduardo de Mello | |
| Author | Rose, Tatiana Lundgren | |
| Author | Silva, Marcelle Figueira Marques da | |
| Author | Fialho, Alexandre Madi | |
| Author | Assis, Rosane Maria S | |
| Author | Andrade, Juliana da Silva Ribeiro de | |
| Author | Sá, Ana Caroline Costa | |
| Author | Zeller, Mark | |
| Author | Heylen, Elisabeth | |
| Author | Matthijnssens, Jelle | |
| Author | Leite, José Paulo Gagliardi | |
| Access date | 2015-06-12T13:57:51Z | |
| Available date | 2015-06-12T13:57:51Z | |
| Document date | 2014 | |
| Citation | GÓMEZ, Mariela Martínez et al. Prevalence and genomic characterization of G2P[4] group A rotavirus strains during monovalent vaccine introduction in Brazil. Infection, Genetics and Evolution, v.28, p.486–494, 2014. | pt_BR |
| ISSN | 1567-1348 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10817 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Title | Prevalence and genomic characterization of G2P[4] group A rotavirus strains during monovalent vaccine introduction in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.meegid.2014.09.012 | pt_BR |
| Abstract | This study aims to: estimate the prevalence of G2P[4] rotaviruses in Brazil between 2001–2011 from
patients with acute gastroenteritis; perform phylogenetic analyses of G2P[4] Brazilian strains (from vaccinated
and non-vaccinated children) based on VP7 and VP8⁄ encoding genes and analyze the antigenic
regions of these proteins comparing with RV1; and assess the full genetic background of eleven selected
Brazilian strains. The G2P[4] detection rate among RVA positive samples was 0/157 in 2001, 3/226 (1.3%)
in 2002, 0/514 in 2003, 0/651 in 2004, 31/344 (9%)/2005, 112/227 (49%)/2006, 139/211 (66%)/2007, 240/
284 (85%)/2008, 66/176 (37.5%)/2009, 367/422 (87%)/2010 and 75/149 (50%)/2011. For the VP7 and VP8⁄
encoding genes, 52 sequences were analyzed and shared up to 99% nucleotide identity with other contemporary
G2P[4] strains detected worldwide, grouping into different clusters. Most differences inside
antigenic epitopes of VP7 and VP8⁄ have been maintained in the G2P[4] Brazilian strains along the years,
and all were present before RV1 introduction. Eleven G2P[4] strains (4-vaccinated/7-non-vaccinated)
were completely characterized and possessed the typical DS-1-like genotype constellation (G2-P[4]-I2-
R2-C2-M2-A2-N2-T2-E2-H2) sharing up to 99% of nucleotide identity with contemporary worldwide
strains. Reassortments between Brazilian G2P[4] human strains were observed. In conclusion, the data
obtained in the current study suggests that implementation of RV1 vaccination might not influence
the genetic diversity observed in G2P[4] analyzed strains. Several factors might have contributed to
the increased prevalence of this genotype in Brazil since 2005: the introduction of RV1 into the Brazilian
National Immunization Program has resulted in a decrease in the relative prevalence of predominant
Wa-like RVA strains facilitating the increase of the heterotypic (DS-1-like) RVA strain G2P[4] in the
Brazilian population; the genetic diversity found in different geographical regions throughout the years
before, and after the introduction of RV1; the long period of low or no circulation of this genotype in
Brazil previous to RV1 introduction could have created favorable conditions for the accumulation of
immunological susceptible individuals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology.Laboratory of Clinical and Epidemiological Virology. Leuven, Belgium. | pt_BR |
| Affilliation | University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology.Laboratory of Clinical and Epidemiological Virology. Leuven, Belgium. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Acute gastroenteritis | pt_BR |
| Subject | Group A rotaviruses | pt_BR |
| Subject | Genomic background | pt_BR |
| Subject | Monovalent vaccine | pt_BR |
| Subject | G2P[4] | pt_BR |
| DeCS | Gastroenterite | pt_BR |
| DeCS | Rotavirus | pt_BR |
| DeCS | Vacinas | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
