| Author | Vega-Rodriguez, Joel | |
| Author | Ghosh, Anil K | |
| Author | Kanzok, Stefan M | |
| Author | Dinglasan, Rhoel R | |
| Author | Wang, Sibao | |
| Author | Bongio, Nicholas J | |
| Author | Kalume, Dario E | |
| Author | Miura, Kazutoyo | |
| Author | Long, Carole A | |
| Author | Pandey, Akhilesh | |
| Author | Lorena, Marcelo Jacobs | |
| Access date | 2015-06-17T12:05:19Z | |
| Available date | 2015-06-17T12:05:19Z | |
| Document date | 2014 | |
| Citation | VEGA-RODRIGUEZ, Joel et al. Multiple pathways for Plasmodium ookinete invasion of the mosquito midgut. Proc Natl Acad Sci U S A., v.111,, n.4, p. E492–E500, jan. 2014. | pt_BR |
| ISSN | 1091-6490 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10862 | |
| Language | eng | pt_BR |
| Publisher | National Academy of Sciences | pt_BR |
| Rights | open access | |
| Title | Multiple pathways for Plasmodium ookinete invasion of the mosquito midgut | pt_BR |
| Type | Article | |
| DOI | 10.1073/pnas.1315517111 | pt_BR |
| Abstract | Plasmodium ookinete invasion of the mosquito midgut is a crucial
step of the parasite life cycle but little is known about the molecular
mechanisms involved. Previously, a phage display peptide
library screen identified SM1, a peptide that binds to the mosquito
midgut epithelium and inhibits ookinete invasion. SM1 was characterized
as a mimotope of an ookinete surface enolase and SM1
presumably competes with enolase, the presumed ligand, for
binding to a putative midgut receptor. Here we identify a mosquito
midgut receptor that binds both SM1 and ookinete surface enolase,
termed “enolase-binding protein” (EBP). Moreover, we determined
that Plasmodium berghei parasites are heterogeneous for
midgut invasion, as some parasite clones are strongly inhibited by
SM1 whereas others are not. The SM1-sensitive parasites required
the mosquito EBP receptor for midgut invasion whereas the SM1-
resistant parasites invaded the mosquito midgut independently of
EBP. These experiments provide evidence that Plasmodium ookinetes
can invade the mosquito midgut by alternate pathways.
Furthermore, another peptide from the original phage display
screen, midgut peptide 2 (MP2), strongly inhibited midgut invasion
by P. berghei (SM1-sensitive and SM1-resistant) and Plasmodium
falciparum ookinetes, suggesting that MP2 binds to a separate,
universal receptor for midgut invasion. | pt_BR |
| Affilliation | Johns Hopkins University. Bloomberg School of Public Health. Malaria Research Institute. The W. Harry Feinstone Department of Molecular Microbiology and Immunology. Baltimore, MD, USA. | pt_BR |
| Affilliation | Johns Hopkins University. Bloomberg School of Public Health. Malaria Research Institute. The W. Harry Feinstone Department of Molecular Microbiology and Immunology. Baltimore, MD, USA / Case Western Reserve University. Center for Global Health and Diseases. Cleveland, OH, USA. | pt_BR |
| Affilliation | Loyola University. Department of Biology. Chicago, IL, USA. | pt_BR |
| Affilliation | Johns Hopkins University. Bloomberg School of Public Health. Malaria Research Institute. The W. Harry Feinstone Department of Molecular Microbiology and Immunology. Baltimore, MD, USA. | pt_BR |
| Affilliation | Chinese Academy of Sciences. Shanghai Institutes for Biological Sciences. Institute of Plant Physiology and Ecology. cKey Laboratory of Insect Developmental and Evolutionary Biology. Shangai, China. | pt_BR |
| Affilliation | Duquesne University. Department of Biological Sciences. Pittsburgh, PA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. fLaboratory of Malaria and Vector Research. Rockville, MD, USA. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. fLaboratory of Malaria and Vector Research. Rockville, MD, USA. | pt_BR |
| Affilliation | Johns Hopkins University. The Johns Hopkins University School of Medicine. McKusick–Nathans Institute of Genetic Medicine. Departments of Biological Chemistry, Pathology, and Oncology. Baltimore, MD, USA. | pt_BR |
| Affilliation | Johns Hopkins University. Bloomberg School of Public Health. Malaria Research Institute. The W. Harry Feinstone Department of Molecular Microbiology and Immunology. Baltimore, MD, USA. | pt_BR |
| Subject | Plasmodium | pt_BR |
| Subject | Plasmodium berghei | pt_BR |
| Subject | Mosquito | pt_BR |
| DeCS | Malária | pt_BR |
| DeCS | Plasmodium berghei | pt_BR |
