Author | Cardillo, Fabíola | |
Author | Nomizo, Auro | |
Author | Postól, Edilberto | |
Author | Mengele Junior, José Orivaldo | |
Access date | 2015-06-18T13:03:27Z | |
Available date | 2015-06-18T13:03:27Z | |
Document date | 2004 | |
Citation | CARDILLO, F. et al. NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection. Medical Science Monitor, v. 10, n. 8, p. BR259-267, 2004. | pt_BR |
ISSN | 1234-1010 | |
URI | https://www.arca.fiocruz.br/handle/icict/10908 | |
Language | eng | pt_BR |
Publisher | Medical Science International Publishing | pt_BR |
Rights | open access | pt_BR |
Title | NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection. | pt_BR |
Type | Article | pt_BR |
Abstract | BACKGROUND: This study evaluated the regulatory function of NK1.1+ cells during Trypanosoma cruzi infection. MATERIAL/METHODS: Both thymectomized (Tx C57Bl/6) and euthymic C57Bl/6 mice (C57Bl/6) were infected intraperitoneally with the Tulahuen strain. NK1.1+ cells were depleted in vivo by anti-NK1.1 mAb. Spleen cells were analyzed by flow cytometry for the expression of CD44 and CD69 on T cells. Supernatants from splenocytes were used to measure nitrite concentration (quantified by Griess reagent). Interleukin 2 and IFN-gamma levels were determined by ELISA. The protocols used herein were approved by the Institutional Committee for Ethics. Student's t or Kruskal-Wallis tests were applied, as indicated. RESULTS: The number of T cells expressing CD69 increased progressively during T. cruzi infection in NK1.1 cell-depleted C57Bl/6 mice. In spite of an increased early T cell activation during infection, the percentage of CD4+ CD44high T cells did not augment in NK1.1 cell-depleted C57Bl/6 mice compared with untreated C57Bl/6 controls. Serum levels of IFN-gamma in anti-NK1.1-treated mice were higher than in non-depleted animals. Con-A-stimulated spleen cell supernatants from NK1.1 cell-depleted animals contained increased levels of IL-2 and nitric oxide (NO) during early infection. CONCLUSIONS: After the first week of infection, NO overproduction and high levels of IFN-gamma in anti-NK1.1-tre-ated C57Bl/6 mice appeared to be related to susceptibility and hyperactivation of peripheral T cells. Finally, this study suggests a novel regulatory function of NK1.1+ cells during T. cruzi infection. Without NK1.1 cells, T lymphocytes are hyperactivated but do not differentiate to effector/memory T cells in infected C57Bl/6 mice. | pt_BR |
Affilliation | University of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, Brasil | pt_BR |
Affilliation | University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil | pt_BR |
Affilliation | University of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil | pt_BR |
Affilliation | University of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, Brasil | pt_BR |
Subject | T. cruzi | pt_BR |
Subject | NK1.1 T cells | pt_BR |
Subject | Regulatory cells | pt_BR |
Subject | IFN-gamma | pt_BR |
Subject | Nitric oxide | pt_BR |
Subject | Interleukin-2 | pt_BR |
DeCS | Antígenos CD44/metabolismo | pt_BR |
DeCS | Antígenos de Diferenciação de Linfócitos T/metabolismo | pt_BR |
DeCS | Células Matadoras Naturais/imunologia | pt_BR |
DeCS | Baço/imunologia | pt_BR |
DeCS | Linfócitos T/imunologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Citometria de Fluxo | pt_BR |
DeCS | Interferon gama/metabolismo | pt_BR |
DeCS | Interleucina-2/metabolismo | pt_BR |
DeCS | Lectinas Tipo C | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos C57BL | pt_BR |
DeCS | Óxido Nítrico/metabolismo | pt_BR |
DeCS | Baço/parasitologia | pt_BR |
DeCS | Trypanosoma cruzi/imunologia | pt_BR |