| Author | Melo, Tatiana G | |
| Author | Tucci, Amanda R | |
| Author | Nogueira, Alanderson R | |
| Author | Meirelles, Maria de Nazareth S. L | |
| Author | Pereira, Mirian C. S | |
| Access date | 2015-06-22T12:26:36Z | |
| Available date | 2015-06-22T12:26:36Z | |
| Document date | 2014 | |
| Citation | MELO, Tatiana G. et al. The involvement of FAK and Src in the invasion of cardiomyocites by Tripanosoma cruzi. Experimental Parasitology, v.139, p.49-57, 2014. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10931 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | The involvement of FAK and Src in the invasion of cardiomyocytes by Trypanosoma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2014.02.008 | pt_BR |
| Abstract | The activation of signaling pathways involving protein tyrosine kinases (PTKs) has been demonstrated
during Trypanosoma cruzi invasion. Herein, we describe the participation of FAK/Src in the invasion of
cardiomyocytes by T. cruzi. The treatment of cardiomyocytes with genistein, a PTK inhibitor, significantly
reduced T. cruzi invasion. Also, PP1, a potent Src-family protein inhibitor, and PF573228, a specific FAK
inhibitor, also inhibited T. cruzi entry; maximal inhibition was achieved at concentrations of 25 lM
PP1 (53% inhibition) and 40 lM PF573228 (50% inhibition). The suppression of FAK expression in siRNA-
treated cells and tetracycline-uninduced Tet-FAK(WT)-46 cells significantly reduced T. cruzi invasion.
The entry of T. cruzi is accompanied by changes in FAK and c-Src expression and phosphorylation. An
enhancement of FAK activation occurs during the initial stages of T. cruzi-cardiomyocyte interaction
(30 and 60 min), with a concomitant increase in the level of c-Src expression and phosphorylation,
suggesting that FAK/Src act as an integrated signaling pathway that coordinates parasite entry. These
data provide novel insights into the signaling pathways that are involved in cardiomyocyte invasion
by T. cruzi. A better understanding of the signal transduction networks involved in T. cruzi invasion
may contribute to the development of more effective therapies for the treatment of Chagas’ disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | FAK | pt_BR |
| Subject | c-Src | pt_BR |
| Subject | Tropanosoma cruzi | pt_BR |
| Subject | Cardiomyocytes | pt_BR |
| Subject | Invasion | pt_BR |
| Subject | Signaling pathway | pt_BR |
| DeCS | Trypanosoma cruzi | pt_BR |
| DeCS | Miócitos Cardíacos | pt_BR |
| DeCS | Doença de Chagas | pt_BR |
