Mostrar el registro sencillo del ítem
| Autor | Diniz, Ivana Márcia Alves | |
| Autor | Teixeira, Karina Imaculada Rosa | |
| Autor | Araújo, Patrícia Valente | |
| Autor | Araújo, Márcio Sobreira Silva | |
| Autor | Marques, Márcia Martins | |
| Autor | Poletto, Luiz Thadeu de Abreu | |
| Autor | Cortés, Maria Esperanza | |
| Fecha de acceso | 2015-06-25T13:23:48Z | |
| Fecha de disponibilización | 2015-06-25T13:23:48Z | |
| Fecha de publicación | 2014 | |
| Referencia | DINIZ, Ivana Márcia Alves et al. Evaluation of antibacterial photodynamictherapy effects on human dental pulp cell cultures. Photodiagnosis Photodyn Thep. Volume 11, fascículo 3, p. 300-3066, 2014 | pt_BR |
| ISSN | 1572-1000 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10964 | |
| Idioma | eng | pt_BR |
| Editor | Elsevier Ltda. | pt_BR |
| Derechos de autor | restricted access | pt_BR |
| Título | Evaluation of antibacterial photodynamictherapy effects on human dental pulp cell cultures | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1016/j.pdpdt.2014.03.010 | |
| Resumen en Inglés | Background: The antibacterial photodynamic therapy (aPDT) has been used in dentistry againstoral microorganisms because of its excellent biocide effect. However, for carious lesions appli-cations, there is little evidence that this therapy is safe for the pulp tissue.Objective: This study evaluates the effects of an aPDT protocol on human pulp cells in vitro.Methods: Pulp cells isolated from dental pulp were exposed to an aPDT protocol associat-ing methylene blue (MB) at concentrations of 0.0125, 0.025 and 0.050 mg/ml and red laserirradiation using a continuous-wave indium-gallium-aluminum-phosphide (InGaAlP) diode laser( _ = 660 nm, 40 mW, 2.4 J, 60 J/cm2for 1 min). Pre-irradiation time was 5 min for each MB con-centration. Cell viability was determined by MTT assay and activity of alkaline phosphatase wasassessed by BCIP-NBT assay. Type of aPDT-induced cell death was assessed by flow cytometry.Data was statistically compared (ANOVA followed by Tukey’ or Bonferroni’s post hoc tests).Results: aPDT was able to kill pulp cells in a dye concentration-dependent manner. The cel-lular viability was significantly reduced when used MB at 0.025 or 0.050 mg/ml concentrations(p < 0.0001). At these concentrations, aPDT-induced cell death occurred mostly by necrosis.Alkaline phosphatase activity was significantly reduced in all experimental groups (p < 0.001).Pulp cells showed suitable viability when MB at 0.0125 mg/ml was exposed to laser irradiation. | pt_BR |
| Afiliación | Universidade Federal de Minas Gerais. Faculdade de Odontologia. Departamento de Odontologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade Federal de Minas Gerais. Faculdade de Odontologia. Departamento de Odontologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade Federal de Minas Gerais. Faculdade de Odontologia. Departamento de Odontologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Fundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade de São Paulo. Faculdade de Odontologia. Departamento de Odontologia. São Paulo, SP, Brazil | pt_BR |
| Afiliación | Universidade Federal de Minas Gerais. Faculdade de Odontologia. Departamento de Odontologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade Federal de Minas Gerais. Faculdade de Odontologia. Departamento de Odontologia. Belo Horizonte, MG, Brazil | pt_BR |
| Palavras clave en Inglês | Antibacterial photodynamictherapy | pt_BR |
| Palavras clave en Inglês | Methylene blue | pt_BR |
| Palavras clave en Inglês | Cytotoxicity | pt_BR |
| Palavras clave en Inglês | Dental pulp cell | pt_BR |
| Palavras clave en Inglês | Red laser | pt_BR |
Ficheros en el ítem
