| Author | Victoria, M | |
| Author | Fumian, T. M | |
| Author | Rocha, M. S | |
| Author | Leite, J. P. G. | |
| Author | Girones, R | |
| Author | Miagostovich, M. P | |
| Access date | 2015-07-01T12:12:30Z | |
| Available date | 2015-07-01T12:12:30Z | |
| Document date | 2014 | |
| Citation | VICTORIA, M. et al. Gastroenteric virus dissemination and influence of rainfall events in urban beaches in Brazil. Journal of Applied Microbiology, v.117, n.4, p.1210-1218, oct. 2014. | pt_BR |
| ISSN | 1364-5072 | pt_BR |
| ISSN | 10.1111/jam.12592 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11044 | |
| Language | eng | pt_BR |
| Publisher | Wiley OnlIne Library | pt_BR |
| Rights | restricted access | |
| Title | Gastroenteric virus dissemination and influence of rainfall events in urban beaches in Brazil | pt_BR |
| Type | Article | |
| Abstract | Aims
This study was conducted to assess rotavirus A (RV-A), genogroup II (GII) norovirus (NoV), and human adenovirus (HAdV) dissemination in recreational water in an urban beach located in the city of Rio de Janeiro and their persistence during rainfall events.
Methods and Results
Viruses, including bacteriophage (PP7), used as internal control, were concentrated, reverse transcribed and quantified by a low-cost method based on organic flocculation with skimmed milk coupled with quantitative polymerase chain reaction protocols. The analysis of 74 superficial water samples obtained during 6 months of monitoring detected HAdV (66%), RV-A (37%) and GII NoV (14%), with a mean viral load of 4·1 log10 genome copies l−1 (g.c. l−1), 4·3 log10 g.c l−1 and 3·8 log10 g.c. l−1, respectively. Investigation of those viruses during two rainfall events showed a longer permanence after rainfall events compared with bacterial indicators.
Conclusions
The results highlight the need for further monitoring using viral parameters to determine the microbiological quality of recreational waters to allow bath in these waters, especially during rainy events.
Significance and Impact of the Study
This study provides data on virus contamination in recreational waters on tourist beaches frequented throughout the year, emphasizing the importance of viral parameters for assessing microbiological quality of water, as well as the potential risk of waterborne infections. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil / Universidad de la Republica. Regional Norte. Laboratorio de Virologia Comparativa e Molecular. Salto, Uruguay. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad de la Republica. Regional Norte. Departamento de Matemática e Estatística. Salto, Uruguay. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Barcelona. Department of Microbiology. Laboratory of Water and Food Viral Contaminants. Barcelona, Spain. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Beach | pt_BR |
| Subject | Gastroenteric viruses | pt_BR |
| Subject | Rainfall | pt_BR |
| Subject | Recreational water | pt_BR |
| Subject | Quantification | pt_BR |
| DeCS | Precipitação Atmosférica | pt_BR |
| DeCS | Água para Recreação | pt_BR |
| DeCS | Praias | pt_BR |
| DeCS | Gastroenterite | pt_BR |
| DeCS | Virus da Gastroenterite Transmissível | pt_BR |
