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| Autor | Artico, Sinara | |
| Autor | Alves, Marcelo Ribeiro | |
| Autor | Oliveira Neto, Osmundo Brilhante | |
| Autor | Macedo, Leonardo Lima Pepino de | |
| Autor | Silveira, Sylvia | |
| Autor | Sa, Maria Fátima Grossi de | |
| Autor | Martinelli, Adriana Pinheiro | |
| Autor | Ferreira, Marcio Alves | |
| Fecha de acceso | 2015-07-01T18:37:30Z | |
| Fecha de disponibilización | 2015-07-01T18:37:30Z | |
| Fecha de publicación | 2014 | |
| Referencia | ARTICO, Sinara et al. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae. BMC Genomics, v.15, n.854, p.1-24, 2014. | pt_BR |
| ISSN | 1471-2164 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11051 | |
| Promoción | We thank Prof. S.M. Tsai, Cell and Molecular Biology Laboratory, CENA/USP, for maintaining the multiuser LMD facility (FAPESP 2009/53998-3). APM acknowledges CNPq (Grant 310.612/2011-0). The authors gratefully thank Marco A. Takita and Carolina M. Rodrigues for use of the computational structure in the Laboratory of Biotechnology, Center for Citrus Sylvio Moreira, Cordeirópolis-Sao Paulo, Brazil. We thank Julia Lambret-Frotté for help us with the phylogenetic analysis. This work was part of S.A’s Ph. D. research in Genetics at the Department of Genetics of the Universidade Federal do Rio de Janeiro (UFRJ) and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; M. Alves-Ferreira: # 306025/2010-8) and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ). | pt_BR |
| Idioma | eng | pt_BR |
| Editor | BMC Genomics | pt_BR |
| Derechos de autor | open access | pt_BR |
| Palabras clave en Portugués | Larva | pt_BR |
| Palabras clave en Portugués | Sequenciamento Transcriptoma | pt_BR |
| Palabras clave en Portugués | Estresse Biótico | pt_BR |
| Título | Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae | pt_BR |
| Tipo del documento | Article | pt_BR |
| DOI | 10.1186/1471-2164-15-854 | |
| Resumen en Inglés | Background: Cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (Anthonomus grandis). Transcriptome analysis was performed to understand the molecular interactions between Gossypium hirsutum L. and cotton boll weevil larvae. The Illumina HiSeq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae. Results: The analysis generated a total of 327,489,418 sequence reads that were aligned to the G. hirsutum reference transcriptome. The total number of expressed genes was over 21,697 per sample with an average length of 1,063 bp. The DEGseq analysis identified 443 differentially expressed genes (DEG) in cotton flower buds infected with boll weevil larvae. Among them, 402 (90.7%) were up-regulated, 41 (9.3%) were down-regulated and 432 (97.5%) were identified as orthologues of A. thaliana genes using Blastx. Mapman analysis of DEG indicated that many genes were involved in the biotic stress response spanning a range of functions, from a gene encoding a receptor-like kinase to genes involved in triggering defensive responses such as MAPK, transcription factors (WRKY and ERF) and signalling by ethylene (ET) and jasmonic acid (JA) hormones. Furthermore, the spatial expression pattern of 32 of the genes responsive to boll weevil larvae feeding was determined by “in situ” qPCR analysis from RNA isolated from two flower structures, the stamen and the carpel, by laser microdissection (LMD). Conclusion: A large number of cotton transcripts were significantly altered upon infestation by larvae. Among the changes in gene expression, we highlighted the transcription of receptors/sensors that recognise chitin or insect oral secretions; the altered regulation of transcripts encoding enzymes related to kinase cascades, transcription factors, Ca2+ influxes, and reactive oxygen species; and the modulation of transcripts encoding enzymes from phytohormone signalling pathways. These data will aid in the selection of target genes to genetically engineer cotton to control the cotton boll weevil. | pt_BR |
| Afiliación | Universidade Federal do Rio de Janeiro. Departamento de Genetica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil | pt_BR |
| Afiliación | Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF, Brasil / Escola de Medicina Faciplac. Departamento de Bioquímica e Biologia Molecular. Brasília, DF, Brasil | pt_BR |
| Afiliación | Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF, Brasil | pt_BR |
| Afiliación | Universidade de São Paulo. Piracicaba, SP, Brasil | pt_BR |
| Afiliación | Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF, Brasil / Universidade Católica de Brasília. Brasília, DF, Brasil | pt_BR |
| Afiliación | Universidade de São Paulo. Piracicaba, SP, Brasil | pt_BR |
| Afiliación | Universidade Federal do Rio de Janeiro. Departamento de Genetica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Palavras clave en Inglês | Transcriptome | pt_BR |
| Palavras clave en Inglês | Laser Capture Microdissection | pt_BR |
| Palavras clave en Inglês | Gossypium | pt_BR |
| Palavras clave en Inglês | RNA | pt_BR |
| Palavras clave en Inglês | Larvae | pt_BR |
| Palavras clave en Inglês | Transcriptome Sequencing | pt_BR |
| Palavras clave en Inglês | Biotic Stress | pt_BR |
| DeCS | Transcriptoma | pt_BR |
| DeCS | Microdissecção e Captura a Laser | pt_BR |
| DeCS | Gossypium | pt_BR |
| DeCS | RNA | pt_BR |
| e-ISSN | 1471-2164 |
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