| Author | Silva, Dolores | |
| Author | Ponte, Carlos G. G. | |
| Author | Hacker, Mariana A. | |
| Author | Antas, Paulo R. Z. | |
| Access date | 2015-07-03T12:34:37Z | |
| Available date | 2015-07-03T12:34:37Z | |
| Document date | 2013 | |
| Citation | SILVA, Dolores et al. A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens. Acta Tropica, v.127, n.2, p.85-81, aug. 2013. | pt_BR |
| ISSN | 0001-706X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11064 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.actatropica.2013.04.002 | pt_BR |
| Abstract | In vitro stimulation of whole blood or isolated peripheral blood cells with specific antigens is used for several purposes. We sought to identify a reliable, reproducible, fast and feasible in vitro method to assess human cellular immune responses to Mycobacterium tuberculosis. In contrast to peripheral blood mononuclear cell (PBMC) culture, a whole blood assay (WBA) provides a more physiological environment, which may provide a broader assessment of serum biomarker, biosignature profiles. Twenty-three asymptomatic individuals with M. tuberculosis infection were recruited. Total cells from the WBA (diluted 1:3 in completed RPMI) and PBMC (2 × 105 cells/ml) plus M. tuberculosis Ag85A, Ag85B, ESAT-6 and Mycobacterium bovis 65 kDa were characterized by flow cytometry, then added in 96-well plates and on day 5 plasma and supernatants were harvested for detection of 17 cytokines by a Luminex array system. There was agreement between PBMC and WBA for IL-2, IL-5, IL-6, IL-7, IL-13, IFN-γ, TNF-α, MCP-1 and MIP-1β. There was evidence toward higher IL-10 (p ≤ 0.049) and G-CSF (p ≤ 0.012) plasma production, and higher IL-1β (p ≤ 0.048), IL-4 (p ≤ 0.044), IL-12p70 (p ≤ 0.006), IL-17 (p ≤ 0.002) and GM-CSF (p ≤ 0.049) production for PBMC vs. WBA. Both methods provided virtually no reaction to the internal, negative control. Due to technical issues linked to data out of range, IL-8 data were not considered. These results suggest that, depending on the method employed, PBMC and/or WBA techniques provide fine conditions for the model proposed and thus whole blood cultures are well-suited low-cost proxy-measures during search for serum biomarkers. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Vanderbilt University School of Medicine. Department of Medicine. Division of Infectious Diseases. Nashville, USA. | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Chemokines | pt_BR |
| Subject | Mycobacterium tuberculosis antigens | pt_BR |
| Subject | Whole blood assays | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Cellularity | pt_BR |
| Subject | Serum biomarkers | pt_BR |
| DeCS | Tuberculose | pt_BR |
| DeCS | Biomarcadores | pt_BR |
| DeCS | Antígenos | pt_BR |
| DeCS | Quimiocinas | pt_BR |
| DeCS | Citocinas | pt_BR |
| DeCS | Mycobacterium tuberculosis | pt_BR |
