| Author | Lima, Tatjana Keesen de Souza | |
| Author | Estanislau, Juliana de Assis Silva Gomes | |
| Author | Gusmão, Rafaelle Christine Gomes Fares | |
| Author | Araujo, Fernanda Fortes | |
| Author | Ferreira, Karine Silvestre | |
| Author | Chaves, Ana Thereza | |
| Author | Rocha, Manoel Otávio da Costa | |
| Author | Oliveira, Rodrigo Correa de | |
| Access date | 2015-08-05T17:06:59Z | |
| Available date | 2015-08-05T17:06:59Z | |
| Document date | 2012 | |
| Citation | KEESEN, Tatjana de Souza Lima et al. Characterization of CD4⁺ cytotoxic lymphocytes and apoptosis markers induced by Trypanossoma cruzi infection. Scandinavian Journal Immunology, vol.76, pp. 311-319, 2012 | pt_BR |
| ISSN | 0300-9475 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11424 | |
| Language | eng | pt_BR |
| Publisher | Blackwell Scientific Publications | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Characterization of CD4⁺ cytotoxic lymphocytes and apoptosis markers induced by Trypanossoma cruzi infection | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1365-3083.2012.02730.x | |
| Abstract | Although the pathophysiology of Chagas disease is not completely understood, it is widely accepted that involvement of the immune response is critical in determining the outcome of the disease. In this context, CD4⁺ T cells may play an important role in generating different mechanisms of protection. In addition to effector and regulatory functions, CD4⁺ T cells may be also involved with lytic activities against the parasite and may have a relevant role on control of the infection. In this study, we have evaluated CD4⁺ T cells expressing cytotoxic and apoptosis markers in response to Trypanossoma cruzi infection in indeterminate (IND) and cardiac (CARD) patients with Chagas disease and non-infected individuals (NI). Our data demonstrated that: (1) CD4⁺ T cells presented higher ex vivo granzyme B expression in patients with Chagas disease compared with healthy individuals and that antigen induced a greater granzyme B expression in IND patients; (2) CD95L expression in CD4⁺ CD95⁺ T cells from IND patients is higher than in CARD and NI; (3) IND and CARD patients had an increased frequency of caspase-3 after in vitro stimulation and also expressed a high frequency of annexinV⁺ 7ADD⁺ within CD4⁺ T cells; (4) Lastly, a positive correlation was seen between cytotoxic molecules and CD45RO memory marker in CD4⁺ T cells and between caspase-3 and CD95L within CD4⁺ CD95⁺ T cells. These results suggest new insights into the functional competence of CD4⁺ T cells among the different clinical forms of Chagas disease, which will lead to a better understanding of their influence during immune responses against T. cruzi. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Natal, RN, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Infectologia e Medicina Tropical. Belo Horizonte, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Subject | Biological Markers/analysis | pt_BR |
| Subject | Cytotoxicity | pt_BR |
| Subject | Immunologic/immunology | pt_BR |
| Subject | T-Lymphocyte Subsets/immunology | pt_BR |
| Subject | Lysosomal-Associated Membrane Protein 1 | pt_BR |
