| Author | Casteluber, Marisa Cristina Fonseca | |
| Author | Damasceno, Leonardo Miranda | |
| Author | Silveira, Wendel Batista | |
| Author | Diniz, Raphael Hermano Santos | |
| Author | Passos, Frederico Jose Vieira | |
| Author | Passos, Flavia Maria Lopes | |
| Access date | 2015-08-19T12:12:05Z | |
| Available date | 2015-08-19T12:12:05Z | |
| Document date | 2012 | |
| Citation | CASTELUBER, Marisa Cristina Fonseca et al. Cloning and expression of a functional core streptavidin in Pichia pastoris: Strategies to increase yield. Biotechnology Progress, vol.28, n. 6, p. 1419–1425, 2012. | pt_BR |
| ISSN | 8756-7938 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11455 | |
| Language | eng | pt_BR |
| Publisher | Wiley-Blackwell | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Cloning and expression of a functional core streptavidin in Pichia pastoris: Strategies to increase yield | pt_BR |
| Type | Article | |
| DOI | 10.1002/btpr.1621 | |
| Abstract | Streptavidin is widely used as an analytical tool and affinity tag together with biotinylated surfaces or molecules. We report for the first time a simple strategy that yields high biomass of a Pichia pastoris strain containing a methanol induced core streptavidin (cStp) gene. Three factors were evaluated for biomass production: glycerol concentration, aeration, and feed flow rates in a bioreactor. Recycling of recombinant cells, either free or immobilized, was investigated during induction. Concentration of 2.0 M glycerol, feeding flow rate of 0.11 mL min(-1) , and aeration by air injection dispersed with a porous stone combined with agitation at 500 rpm were the set of conditions resulting into maximum biomass yield (150 g L(-1) ). These parameters yielded 4.0 g L(-1) of cStp, after 96 h of induction. Recombinant biomass was recycled twice before being discarded, which can reduce production costs and simplify the process. Immobilized P. pastoris biomass produced 2.94 and 1.70 g L(-1) of cStp in the first and second induction cycle, respectively. Immobilization and recycling of recombinant P. pastoris biomass opens new possibilities as a potential strategy to improve volumetric productivity for heterologous protein expression. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Tecnologia de Alimentos. Viçosa, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil | pt_BR |
| Subject | Pichia pastoris | pt_BR |
| Subject | streptadivin | pt_BR |
| Subject | heterologous protein expression | pt_BR |
| Subject | recycling of biomass | pt_BR |
| Subject | aeration | pt_BR |
| xmlui.metadata.dc.subject.ods | 12 Consumo e produção responsáveis | |
