| Author | Silva, Katina R. | |
| Author | Liechocki, Sally | |
| Author | Carneiro, João R. | |
| Author | Silva, Cesar Claudio da | |
| Author | Monteiro, Clarissa M. Maya | |
| Author | Borojevic, Radovan | |
| Author | Baptista, Leandra S. | |
| Access date | 2015-08-19T13:49:20Z | |
| Available date | 2015-08-19T13:49:20Z | |
| Document date | 2015 | |
| Citation | SILVA, Karina R.; et al. Stromal-vascular fraction content and adipose stem cell behavior are altered in morbid obese and post bariatric surgery ex-obese women. Stem Cell Research & Therapy, v.6:72, 13p, 2015. | pt_BR |
| ISSN | 1574-888X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11486 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Title | Stromal-vascular fraction content and adipose stem cell behavior are altered in morbid obese and post bariatric surgery ex-obese women | pt_BR |
| Type | Article | |
| DOI | 10.1186/s13287-015-0029-x | pt_BR |
| Abstract | Introduction: Subcutaneous adipose tissue is an interesting source of autologous stem cells with a fundamental
role in the pathophysiology of obesity, metabolic syndromes and insulin resistance. We hypothesize that obesity
could alter the stromal-vascular fraction (SVF) and adipose stem cell (ASCs) functions, which could compromise its
regenerative behavior. Furthermore, we aimed to evaluate whether ASCs derived from post bariatric surgery ex-obese
women maintain their functions in a similar fashion as do those from individuals who have never been obese.
Methods: The SVF of subcutaneous adipose tissue from control (n = 6, body mass index – BMI - 27.5 ± 0.5 kg/m2),
obese (n = 12, BMI 46.2 ± 5.1 kg/m2) and post bariatric surgery ex-obese (n = 7, initial BMI 47.8 ± 1.3 kg/m2; final BMI
28.1 ± 1.1 kg/m2) women were isolated and evaluated by flow cytometry. ASCs were tested for lipid accumulation by
perilipin, adipose differentiation-related protein (ADRP) and Oil Red O staining after adipogenic stimulus. The cytokines
secreted by the ASCs and after lipid accumulation induction were also evaluated.
Results: The subcutaneous adipose tissue of obese and post bariatric surgery ex-obese women was enriched in
pericytes (p = 0.0345). The number of supra-adventitial cells was not altered in the obese patients, but it was highly
enriched in the post bariatric surgery ex-obese women (p = 0.0099). The ASCs of the post bariatric surgery ex-obese
patients secreted more MCP-1 (monocyte chemoattractant protein-1; p = 0.0078). After lipid accumulation induction,
the ASCs of the patients in all groups secreted less IL-6 than the ASCs with no adipogenic stimulus (p < 0.0001). Obese
ASCs with lipid accumulation secreted the highest amount of IL-6 (p < 0.001) whereas the ASCs from the controls
secreted the highest amount of adiponectin (p < 0.0001). The ASCs from the post bariatric surgery ex-obese patients
showed the highest levels of lipid accumulation whereas those from the obese women had the lowest levels (p < 0.0001).
Conclusions: SVF content and ASC behavior are altered in the subcutaneous adipose tissue of morbid obese
women; these changes are not completely restored after bariatric surgery-induced weight loss. The cellular
alterations described in this study could affect the regenerative effects of adipose stem cells. Further investigations are
required to avoid jeopardizing the development of autologous stem cell-based therapies. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Clínica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Xerém em Biologia (Numpex-Bio). Duque de Caxias, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro). Diretoria de Programas. Programa de Engenharia. Duque de Caxias, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro.Hospital Universitário Clementino Fraga Filho. Departamento de Nutrologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro.Hospital Universitário Clementino Fraga Filho. Serviço de Cirurgia Plástica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Clínica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro). Diretoria de Programas. Programa de Bioengenharia. Duque de Caxias, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Clínica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Xerém em Biologia (Numpex-Bio). Duque de Caxias, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro). Diretoria de Programas. Programa de Engenharia. Duque de Caxias, RJ, Brasil. | pt_BR |
| Subject | Post bariatric surgery | pt_BR |
| Subject | Ex-obese women | pt_BR |
| Subject | Adipose stem cell behavior | pt_BR |
| Subject | Morbid obese | pt_BR |
| Subject | Pathophysiology | pt_BR |
| DeCS | Tecido Adiposo | pt_BR |
| DeCS | Obesidade | pt_BR |
| DeCS | Células-Tronco | pt_BR |
| DeCS | Mulheres | pt_BR |
| xmlui.metadata.dc.subject.ods | 05 Igualdade de gênero | |
