| Author | Nussbaum, Claudia | |
| Author | Bannenberg, Sarah | |
| Author | Keul, Petra | |
| Author | Gräler, Markus H. | |
| Author | Albuquerque, Cassiano F. Gonçalves de | |
| Author | Korhonen, Hanna | |
| Author | Lipinski, Karin von Wnuck | |
| Author | Heusch, Gerd | |
| Author | Faria Neto, Hugo C. de Castro | |
| Author | Rohwedder, Ina | |
| Author | Göther, Joachim R. | |
| Author | Prasad, Vysakh Pushpa | |
| Author | Haufe, Günter | |
| Author | Lange-Sperandio, Baerbel | |
| Author | Offermanns, Stefan | |
| Author | Sperandio, Markus | |
| Author | Levkau, Bodo | |
| Access date | 2015-08-19T13:49:20Z | |
| Available date | 2015-08-19T13:49:20Z | |
| Document date | 2015 | |
| Citation | NUSSBAUM, Claudia; et al. Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin. Nature Communications, 6:6416, 12p, 2015. | pt_BR |
| ISSN | 2041-1723 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11487 | |
| Language | eng | pt_BR |
| Publisher | Macmillan Publishers Limited | pt_BR |
| Rights | open access | |
| Title | Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin | pt_BR |
| Type | Article | |
| DOI | 10.1038/ncomms7416 | pt_BR |
| Abstract | Sphingosine-1-phosphate (S1P) participates in inflammation; however, its role in leukocyte rolling is still unclear. Here we use intravital microscopy in inflamed mouse cremaster muscle venules and human endothelial cells to show that S1P contributes to P-selectin-dependent leukocyte rolling through endothelial S1P receptor 3 (S1P3) and Gαq, PLCβ and Ca(2+). Intra-arterial S1P administration increases leukocyte rolling, while S1P3 deficiency or inhibition dramatically reduces it. Mast cells involved in triggering rolling also release S1P that mobilizes P-selectin through S1P3. Histamine and epinephrine require S1P3 for full-scale effect accomplishing it by stimulating sphingosine kinase 1 (Sphk1). In a counter-regulatory manner, S1P1 inhibits cAMP-stimulated Sphk1 and blocks rolling as observed in endothelial-specific S1P1(-/-) mice. In agreement with a dominant pro-rolling effect of S1P3, FTY720 inhibits rolling in control and S1P1(-/-) but not in S1P3(-/-) mice. Our findings identify S1P as a direct and indirect contributor to leukocyte rolling and characterize the receptors mediating its action. | pt_BR |
| Affilliation | Ludwig Maximilians Universität München. Walter Brendel Center, Münche, Germany / Ludwig Maximilians University München. Dr v. Haunersches Children’s Hospital. München, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen. University Hospital Essen. West German Heart and Vascular Center. Institute of Pathophysiology. Essen, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen. University Hospital Essen. West German Heart and Vascular Center. Institute of Pathophysiology. Essen, Germany. | pt_BR |
| Affilliation | University Hospital Jena. Center for Molecular Biomedicine.Center for Sepsis Control and Care. Department of Anesthesiology and Intensive Care Medicine. Jena, Germany. | pt_BR |
| Affilliation | Ludwig Maximilians Universität München. Walter Brendel Center, Münche, Germany / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen. University Hospital Essen. West German Heart and Vascular Center. Institute of Pathophysiology. Essen, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen. University Hospital Essen. West German Heart and Vascular Center. Institute of Pathophysiology. Essen, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Ludwig Maximilians Universität München. Walter Brendel Center, Münche, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen.University Hospital Essen. Department of Hematology. Essen, Germany. | pt_BR |
| Affilliation | Westfälische Wilhelms-Universität Münster. Organisch-Chemisches Institut. Münster, Germany. | pt_BR |
| Affilliation | Westfälische Wilhelms-Universität Münster. Organisch-Chemisches Institut. Münster, Germany. | pt_BR |
| Affilliation | Ludwig Maximilians University München. Dr v. Haunersches Children’s Hospital. München, Germany. | pt_BR |
| Affilliation | Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany. | pt_BR |
| Affilliation | Ludwig Maximilians Universität München. Walter Brendel Center, Münche, Germany. | pt_BR |
| Affilliation | University of Duisburg-Essen. University Hospital Essen. West German Heart and Vascular Center. Institute of Pathophysiology. Essen, Germany. | pt_BR |
| Subject | Endothelial P-selectin | pt_BR |
| Subject | Sphingosine-1-phosphate | pt_BR |
| Subject | Leukocyte | pt_BR |
| DeCS | Selectina-P | pt_BR |
| DeCS | Psicosina | pt_BR |
| DeCS | Leucócitos | pt_BR |
