Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11511
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dc.contributor.authorEsquenazi, Danuza
dc.contributor.authorAlvim, Iris Maria Peixoto
dc.contributor.authorPinheiro, Roberta Olmo
dc.contributor.authorOliveira, Eliane Barbosa de
dc.contributor.authorMoreira, Lilian de Oliveira
dc.contributor.authorSarno, Euzenir Nunes
dc.contributor.authorNery, José Augusto da Costa
dc.date.accessioned2015-08-19T13:49:25Z
dc.date.available2015-08-19T13:49:25Z
dc.date.issued2015
dc.identifier.citationESQUENAZI, Danuza; et al. Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse. Plos One, v. 10, n. 5, p. 1-17, 2015.
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/11511
dc.descriptionCorrection: Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse Danuza Esquenazi, Iris Maria Peixoto Alvim, Roberta Olmo Pinheiro, Eliane Barbosa de Oliveira,Lilian de Oliveira Moreira, Euzenir Nunes Sarno,Jose Augusto da Costa Nery. Notice of Republication This article was republishedon Jul 23,2015, to correctim properly-formatted Greek letters throughout the text. Please download this article again to view the correct version. Reference 1. Esquenazi D,AlvimI M P, Pinheiro R O, Oliveira E Bd, Moreira Ld O,Sarno E N,et al. (2015) Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical presentation of Multibacillary Leprosy Relapse. PLoS ONE doi:10.1371/journal. pone.0127416 PMID:25992795.
dc.language.isoeng
dc.publisherPlos One
dc.rightsopen access
dc.titleCorrelation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
dc.typeArticle
dc.identifier.doi10.1371/journal.pone.0127416
dc.description.abstractenBackground: Despite the efficacy of multidrug therapy, surviving Mycobacterium leprae causes relapse in some leprosy patients, and these patients present signs and symptoms of disease after healing. This study focused on the cellular immune response in relapsed multibacillary patients but also included non-relapsed multibacillary cured individuals, newly diagnosed and untreated multibacillary patients, paucibacillary patients just before the beginning of treatment, and voluntary healthy individuals for comparative analysis.Methodology/Principal Findings Inhibition of CD86 expression in the blood-derived monocytes and dendritic cells of relapsed multibacillary patients, either ex vivo or after M. leprae antigen stimulation was observed by flow cytometry. In addition, no significant changes in Interferon-gamma (IFN-g) expression were observed in 5-day culture supernatants of relapsed patients in response to M. leprae, neither before nor after treatment, as measured by ELISA. However, these patients demonstrated a significant increase in central memory CD4+ and CD8+ M. lepraespecific T cells, as assessed by multiparametric flow cytometry. The increase in frequency of central memory T cells in relapsed patients strongly correlated with the bacillary index and the number of skin lesions observed in these subjects. Moreover, cytokine multiplex analysis demonstrated significant antigen-specific production of Interlukin-1beta (IL-1b), IL-6, and Tumour Necrosis Factor (TNF) in the relapsed group with extremely low IL-10 production, which resulted in a high TNF/IL-10 ratio.Conclusions/Significance Inhibition of CD86 expression may function to reduce effector T cell responses against the M. leprae antigen. Furthermore, the predominance of central memory T cells in association with the high TNF/IL-10 ratio and no observed IFN-g production may be related to the pathogenesis of relapse in multibacillary leprosy. Therefore, our findings may be a direct result of the clinical presentation, including a number of skin lesions and bacterial load, of relapsed patients. To our knowledge, this is the first study correlating immune response parameters with the clinical presentation of relapsed multibacillary patients.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Centro de Ciências Médicas. Departamento de Patologia e Laboratórios. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.subject.enMultibacillary Leprosy
dc.subject.enCentral Memory T Cell Expression
dc.subject.enProinflammatory Cytokine Production
dc.subject.enHanseníase Multibacilar
dc.subject.decsHanseníase Multibacilar
dc.subject.decsCitocina
dc.subject.decsMycobacterium leprae
dc.subject.decsResistência a Múltiplos Medicamentos
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