| Author | Pavan, Márcio G. | |
| Author | Mesquita, Rafael D, | |
| Author | Lawrence, Gena G, | |
| Author | Lazoski, Cristiano | |
| Author | Dotson, Ellen M. | |
| Author | Abubucker, Sahar | |
| Author | Mitreva, Makedonka | |
| Author | Randall-Maher, Jennifer | |
| Author | Monteiro, Fernando A, | |
| Access date | 2015-08-19T13:49:32Z | |
| Available date | 2015-08-19T13:49:32Z | |
| Document date | 2013 | |
| Citation | PAVAN, Márcio G.; et al. A nuclear single-nucleotide polymorphism (SNP) potentially useful for the separation of Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae). Infect Genet Evol., n.14, 19p, March 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11557 | |
| Language | por | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Title | A nuclear single-nucleotide polymorphism (SNP) potentially useful for the separation of Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae) | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.meegid.2012.10.018. | pt_BR |
| Abstract | The design and application of rational strategies that rely on accurate species identification are
pivotal for effective vector control. When morphological identification of the target vector species
is impractical, the use of molecular markers is required. Here we describe a non-coding, singlecopy
nuclear DNA fragment that contains a single-nucleotide polymorphism (SNP) with the
potential to distinguish the important domestic Chagas disease vector, Rhodnius prolixus, from
members of the four sylvatic Rhodnius robustus cryptic species complex. A total of 96 primer
pairs obtained from whole genome shotgun sequencing of the R. prolixus genome (12,626 random
reads) were tested on 43 R. prolixus and R. robustus s.l. samples. One of the seven amplicons
selected (AmpG) presented a SNP, potentially diagnostic for R. prolixus, on the 280th site. The
diagnostic nature of this SNP was then performed on 154 R. prolixus and R. robustus s.l. samples
aimed at achieving the widest possible geographic coverage. The results of a 60% majority rule
Bayesian consensus tree and a median-joining network constructed based on the genetic
variability observed reveal the paraphyletic nature of the R. robustus species complex, with respect to R. prolixus. AmpG region is located in the fourth intron of the Transmembrane protein
165 gene, which seems to be in the R. prolixus X chromosome. Other possible chromosomal
locations of the AmpG region in the R. prolixus genome are also presented and discussed. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Bioinformática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Division of Parasitic Diseases and Malaria. Entomology Branch. Atlanta, GA, USA; | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Biodiversidade Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Division of Parasitic Diseases and Malaria. Entomology Branch. Atlanta, GA, USA; | pt_BR |
| Affilliation | Washington University School of Medicine.The Genome Institute. St. Louis, MO, USA. | pt_BR |
| Affilliation | Washington University School of Medicine.The Genome Institute. St. Louis, MO, USA. | pt_BR |
| Affilliation | Washington University School of Medicine.The Genome Institute. St. Louis, MO, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Rhodnius | pt_BR |
| Subject | cryptic species | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | nuclear DNA | pt_BR |
| Subject | single-nucleotide polymorphism (SNP) | pt_BR |
| Subject | Hemiptera | pt_BR |
| Subject | Reduviidae | pt_BR |
| DeCS | Rhodnius | pt_BR |
| DeCS | Hemípteros | pt_BR |
| DeCS | Reduviidae | pt_BR |
