| Author | Martins, Mauricio A. | |
| Author | Bonaldo, Myrna C. | |
| Author | Rudersdorf, Richard A. | |
| Author | Piaskowsk, Shari M. | |
| Author | Rakasz, Eva G. | |
| Author | Weisgrau, Kim L. | |
| Author | Furlot, Jessica R. | |
| Author | Eernisse, Christopher M. | |
| Author | Santana, Marlon G. Veloso de | |
| Author | Hidalgo, Bertha | |
| Author | Friedrich, Thomas C. | |
| Author | Chiuchiolo, Maria J. | |
| Author | Parks, Christopher L. | |
| Author | Wilson, Nancy A. | |
| Author | Allison, David B. | |
| Author | Galler, Ricardo | |
| Author | Watkins, David I. | |
| Access date | 2015-08-19T13:49:34Z | |
| Available date | 2015-08-19T13:49:34Z | |
| Document date | 2013 | |
| Citation | MARTINS, Maurício A.; et al. Immunogenicity of Seven New Recombinant Yellow Fever Viruses 17D Expressing Fragments of SIVmac239 Gag, Nef, and Vif in Indian Rhesus Macaques. Plos One, v.8, n.1, 16p, jan. 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11569 | |
| Language | spa | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Title | Immunogenicity of Seven New Recombinant Yellow Fever Viruses 17D Expressing Fragments of SIVmac239 Gag, Nef, and Vif in Indian Rhesus Macaques | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0054434 | pt_BR |
| Abstract | An effective vaccine remains the best solution to stop the spread of human immunodeficiency virus (HIV). Cellular immune
responses have been repeatedly associated with control of viral replication and thus may be an important element of the
immune response that must be evoked by an efficacious vaccine. Recombinant viral vectors can induce potent T-cell
responses. Although several viral vectors have been developed to deliver HIV genes, only a few have been advanced for
clinical trials. The live-attenuated yellow fever vaccine virus 17D (YF17D) has many properties that make it an attractive
vector for AIDS vaccine regimens. YF17D is well tolerated in humans and vaccination induces robust T-cell responses that
persist for years. Additionally, methods to manipulate the YF17D genome have been established, enabling the generation
of recombinant (r)YF17D vectors carrying genes from unrelated pathogens. Here, we report the generation of seven new
rYF17D viruses expressing fragments of simian immunodeficiency virus (SIV)mac239 Gag, Nef, and Vif. Studies in Indian
rhesus macaques demonstrated that these live-attenuated vectors replicated in vivo, but only elicited low levels of SIVspecific
cellular responses. Boosting with recombinant Adenovirus type-5 (rAd5) vectors resulted in robust expansion of SIVspecific
CD8+ T-cell responses, particularly those targeting Vif. Priming with rYF17D also increased the frequency of CD4+
cellular responses in rYF17D/rAd5-immunized macaques compared to animals that received rAd5 only. The effect of the
rYF17D prime on the breadth of SIV-specific T-cell responses was limited and we also found evidence that some rYF17D
vectors were more effective than others at priming SIV-specific T-cell responses. Together, our data suggest that YF17D – a
clinically relevant vaccine vector – can be used to prime AIDS virus-specific T-cell responses in heterologous prime boost
regimens. However, it will be important to optimize rYF17D-based vaccine regimens to ensure maximum delivery of all
immunogens in a multivalent vaccine. | pt_BR |
| Affilliation | University of Miami Miller School of Medicine. Department of Pathology. Miami, FL, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | University of Wisconsin-Madison. Wisconsin National Primate Research Center. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Alabama at Birmingham.Department of Biostatistics. Section on Statistical Genetics. BIrmingham, Alabama< USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / University of Wisconsin-Madison. Department of Pathobiological Sciences. Madison, Wisconsin, USA. | pt_BR |
| Affilliation | International AIDS Vaccine Initiative. AIDS Vaccine Design and Development Laboratory. Brooklyn, NY, USA. | pt_BR |
| Affilliation | International AIDS Vaccine Initiative. AIDS Vaccine Design and Development Laboratory. Brooklyn, NY, USA. | pt_BR |
| Affilliation | University of Alabama at Birmingham.Department of Biostatistics. Section on Statistical Genetics. BIrmingham, Alabama< USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Miami Miller School of Medicine. Department of Pathology. Miami, FL, USA. | pt_BR |
| Subject | Yellow Fever Viruses 17D | pt_BR |
| Subject | HIV | pt_BR |
| Subject | Indian Rhesus Macaques | pt_BR |
| Subject | Immunogenicity | pt_BR |
| DeCS | Febre Amarela | pt_BR |
| DeCS | Vacinas | pt_BR |
| DeCS | HIV | pt_BR |
