| Author | Rodrigues, Igor A. | |
| Author | Azevedo, Mariana M. B. | |
| Author | Chaves, Francisco C. M. | |
| Author | Bizzo, Humberto R. | |
| Author | Côrte-Real, Suzana | |
| Author | Alviano, Daniela S. | |
| Author | Alviano, Celuta S. | |
| Author | Rosa, Maria S. S. | |
| Author | Vermelho, Alane B. | |
| Access date | 2015-08-19T13:49:36Z | |
| Available date | 2015-08-19T13:49:36Z | |
| Document date | 2013 | |
| Citation | RODRIGUES, Igor A.; et al. In vitro cytocidal effects of the essential oil from Croton cajucara (red sacaca) and its major constituent 7- hydroxycalamenene against Leishmania chagasi. BMC Complementary and Alternative Medicine, v.13, n.249, 9p, 2013. | pt_BR |
| ISSN | 1472-6882 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11582 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Title | In vitro cytocidal effects of the essential oil from Croton cajucara (red sacaca) and its major constituent 7- hydroxycalamenene against Leishmania chagasi | pt_BR |
| Type | Article | |
| DOI | 10.1186/1472-6882-13-249 | pt_BR |
| Abstract | Background: Visceral leishmaniasis is the most serious form of leishmaniasis and can be lethal if left untreated. Currently
available treatments for these parasitic diseases are frequently associated to severe side effects. The leaves of Croton
cajucara are used as an infusion in popular medicine to combat several diseases. Previous studies have demonstrated that
the linalool-rich essential oil from C. cajucara (white sacaca) is extremely efficient against the tegumentary specie
Leishmania amazonensis. In this study, we investigated the effects of the 7-hydroxycalamenene-rich essential oil from the
leaves of C. cajucara (red sacaca) against Leishmania chagasi, as well as on the interaction of these parasites with host cells.
Methods: Promastigotes were treated with different concentrations of the essential oil for determination of its minimum
inhibitory concentration (MIC). In addition, the effects of the essential oil on parasite ultrastructure were analyzed by
transmission electron microscopy. To evaluate its efficacy against infected cells, mouse peritoneal macrophages infected
with L. chagasi promastigotes were treated with the inhibitory and sub-inhibitory concentrations of the essential oil.
Results: The minimum inhibitory concentrations of the essential oil and its purified component 7-hydroxycalamenene
against L. chagasi were 250 and 15.6 μg/mL, respectively. Transmission electron microscopy analysis revealed important
nuclear and kinetoplastic alterations in L. chagasi promastigotes. Pre-treatment of macrophages and parasites
with the essential oil reduced parasite/macrophage interaction by 52.8%, while it increased the production of
nitric oxide by L. chagasi-infected macrophages by 80%.
Conclusion: These results indicate that the 7-hydroxycalamenene-rich essential oil from C. cajucara is a promising
source of leishmanicidal compounds. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Programa de Pós Graduação PAPD CAPES/FAPERJ. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. 3Programa de Pós Graduação Ciências dos Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Embrapa Amazônia Ocidental. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Embrapa Agroindústria de Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | In vitro | pt_BR |
| Subject | Leishmania chagasi | pt_BR |
| Subject | 7- hydroxycalamenene | pt_BR |
| Subject | Croton cajucara | pt_BR |
| DeCS | Leishmaniose Visceral | pt_BR |
