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https://www.arca.fiocruz.br/handle/icict/11591
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ArtigoDireito Autoral
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- IOC - Artigos de Periódicos [12642]
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IDENTIFICATION AND CHARACTERIZATION OF HIGHLY DIVERGENT SIMIAN FOAMY VIRUSES IN A WIDE RANGE OF NEW WORLD PRIMATES FROM BRAZIL
Autor(es)
Afiliação
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Centro de Primatologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
nstituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
University of Allahabad. Department of Biochemistry. Allahabad, India.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Centro de Primatologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
nstituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
University of Allahabad. Department of Biochemistry. Allahabad, India.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Center for Disease Control and Prevention. Division of HIV/AIDS Prevention. Atlanta, Georgia, USA.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Programa de Genética. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
Foamy viruses naturally infect a wide range of mammals, including Old World (OWP) and New World primates (NWP), which
are collectively called simian foamy viruses (SFV). While NWP species in Central and South America are highly diverse, only
SFV from captive marmoset, spider monkey, and squirrel monkey have been genetically characterized and the molecular
epidemiology of SFV infection in NWPs remains unknown. We tested a large collection of genomic DNA (n = 332)
comprising 14 genera of NWP species for the presence of SFV polymerase (pol) sequences using generic PCR primers.
Further molecular characterization of positive samples was carried out by LTR-gag and larger pol sequence analysis. We
identified novel SFVs infecting nine NWP genera. Prevalence rates varied between 14–30% in different species for which at
least 10 specimens were tested. High SFV genetic diversity among NWP up to 50% in LTR-gag and 40% in pol was revealed
by intragenus and intrafamilial comparisons. Two different SFV strains infecting two captive yellow-breasted capuchins did
not group in species-specific lineages but rather clustered with SFVs from marmoset and spider monkeys, indicating
independent cross-species transmission events. We describe the first SFV epidemiology study of NWP, and the first evidence
of SFV infection in wild NWPs. We also document a wide distribution of distinct SFVs in 14 NWP genera, including two novel
co-speciating SFVs in capuchins and howler monkeys, suggestive of an ancient evolutionary history in NWPs for at least 28
million years. A high SFV genetic diversity was seen among NWP, yet these viruses seem able to jump between NWP species
and even genera. Our results raise concerns for the risk of zoonotic transmission of NWP SFV to humans as these primates
are regularly hunted for food or kept as pets in forest regions of South America.
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