| Author | Andrade, Josiana G. | |
| Author | Iñiguez, Alena M. | |
| Author | Souza, Adriane N. | |
| Author | Marques, Valéria C. L. | |
| Author | Souza Filho, Gonçalo A. de | |
| Author | Santos, Clóvis P. | |
| Author | DaMAtta, Renato A. | |
| Access date | 2015-08-19T13:49:41Z | |
| Available date | 2015-08-19T13:49:41Z | |
| Document date | 2013 | |
| Citation | ANDRADE, Josiana G.; et al. Genetic characterization of the blood-sucking nematodes Libyostrongylus dentatus and Libyostrongylus douglassii supports their different evolutionary history. Veterinary Parasitology, v.193, n.1-3, p.193-199, March 2013. . | pt_BR |
| ISSN | 0304-4017 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11613 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | Genetic characterization of the blood-sucking nematodes Libyostrongylus dentatus and Libyostrongylus douglassii supports their different evolutionary history | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetpar.2012.11.024 | pt_BR |
| Abstract | Libyostrongylus sp. are nematodes that infect ostriches. Libyostrongylus douglassii was first
described in ostriches from several countries in the world. Later Libyostrongylus dentatus
was morphologically identified in ostriches in the USA and Brazil, and mixed infection is
common in the latter country. The internal transcribed spacer (ITS) region of the ribosomal
DNA gene is used for genetic variability assessment and phylogenetic reconstruction for
many organisms. Through genetic analysis the status of different species morphologically
defined was confirmed and a molecular method was developed to differentiate both species.
ITS1, 5.8S, ITS2 regions of L. douglassii and L. dentatus were characterized. Regarding complete
ITS region, the K2-p genetic distance between the species was 0.060 (SE 0.008) and the
intra-specific distance was 0.002 (SE 0.001) for L. dentatus and 0.006 (SE 0.002) for L. douglassii.
NJ and MP phylogenetic analysis of ITS1 and ITS2 regions indicated that both species
belong to the Trichostrongylidae family, and are evolutionarily different, suported by high
bootstrap value. Based on ITS DNA polymorphisms, a molecular approach was designed to
detect both species. These results are the first molecular characterization of L. douglassii
and L. dentatus, and provide new tools for the identification of these parasites of veterinary
importance. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biologia Celular e Tecidual. Campos de Goytacazes, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Kaboratório de Biotecnologia. Campos de Goitacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Kaboratório de Biotecnologia. Campos de Goitacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Kaboratório de Biotecnologia. Campos de Goitacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biologia Celular e Tecidual. Campos de Goytacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biologia Celular e Tecidual. Campos de Goytacazes, RJ, Brasil. | pt_BR |
| Subject | Libyostrongylus douglassii | pt_BR |
| Subject | Libyostrongylus dentatus | pt_BR |
| Subject | Internal Transcribed Spacer | pt_BR |
| Subject | Genetic diagnosis | pt_BR |
| Subject | Nematodes | pt_BR |
| Subject | Struthio camelus | pt_BR |
| DeCS | Struthioniformes | pt_BR |
