| Author | Rochael, Natalia Cadaxo | |
| Author | Lima, Luize Gonçalves | |
| Author | Oliveira, Sandra Maria Pereira de | |
| Author | Barcinski, Marcello André | |
| Author | Saraiva, Elvira Maria | |
| Author | Monteiro, Robson Queiroz | |
| Author | Silva, Lucia Helena Pinto da | |
| Access date | 2015-08-19T13:49:41Z | |
| Available date | 2015-08-19T13:49:41Z | |
| Document date | 2013 | |
| Citation | ROCHAEL, Natalia Cadaxo; et al. Leishmania amazonensis exhibits phosphatidylserine-dependent procoagulant activity, a process that is counteracted by sandfly saliva. Mem Inst Oswaldo Cruz,v.108, n.6, p.679-685, Sept. 2013. | pt_BR |
| ISSN | 1678-8060 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11618 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Title | Leishmania amazonensis exhibits phosphatidylserine-dependent procoagulant activity, a process that is counteracted by sandfly saliva | pt_BR |
| Type | Article | |
| DOI | 10.1590/0074-0276108062013002 | pt_BR |
| Abstract | Leishmania parasites expose phosphatidylserine (PS) on their surface, a process that has been associated with regulation of host’s immune responses. In this study we demonstrate that PS exposure by metacyclic promastigotes of Leishmania amazonensis favours blood coagulation. L. amazonensis accelerates in vitro coagulation of human plasma. In addition, L. amazonensis supports the assembly of the prothrombinase complex, thus promoting thrombin formation. This process was reversed by annexin V which blocks PS binding sites. During blood meal, Lutzomyia longipalpis sandfly inject saliva in the bite site, which has a series of pharmacologically active compounds that inhibit blood coagulation. Since saliva and parasites are co-injected in the host during natural transmission, we evaluated the anticoagulant properties of sandfly saliva in counteracting the procoagulant activity of L. amazonensis. Lu. longipalpis saliva reverses plasma clotting promoted by promastigotes. It also inhibits thrombin formation by the prothrombinase complex assembled either in phosphatidylcholine (PC)/PS vesicles or in L. amazonensis. Sandfly saliva inhibits factor X activation by the intrinsic tenase complex assembled on PC/PS vesicles and blocks factor Xa catalytic activity. Altogether our results show that metacyclic promastigotes of L. amazonensis are procoagulant due to PS exposure. Notably, this effect is efficiently counteracted by sandfly saliva. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil. | pt_BR |
| Subject | Sandfly saliva | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Phosphatidylserine | pt_BR |
| Subject | Blood coagulation | pt_BR |
| Subject | Lutzomyia longipalpis | pt_BR |
| DeCS | Leishmania amazonensis | pt_BR |
| DeCS | CDPdiacilglicerol-Serina O-Fosfatidiltransferase | pt_BR |
| DeCS | Coagulação Sanguínea | pt_BR |
