| Author | Castro, Márcia Gonçalves de | |
| Author | Nogueira, Fernanda Bruycker de | |
| Author | Nogueira, Rita Maria Ribeiro | |
| Author | Oliveira, Ricardo Lourenço de | |
| Author | Santos, Flávia Barreto dos | |
| Access date | 2015-09-21T17:25:33Z | |
| Available date | 2015-09-21T17:25:33Z | |
| Document date | 2013 | |
| Citation | CASTRO, Márcia Gonçalves de; et al. Genetic variation in the 3’ untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in Brazil. Virology Journal, v.10, n.3, 11p, Jan. 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11737 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Title | Genetic variation in the 3’ untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1186/1743-422x-10-3 | pt_BR |
| Abstract | Background: Dengue, a mosquito-borne viral infection caused by one of the four dengue virus (DENV) serotypes
(DENV-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections
throughout tropical and subtropical regions of the world. In Brazil, the disease has become a major public health
problem and the introduction of DENV-3 in 2000 in Rio de Janeiro (RJ) was associated with severe dengue
epidemics. The potential emergence of strains associated with severe disease highlights the need for the
surveillance of DENV in human host and vectors.
Methods: Aiming to contribute for DENV phylogenetic and vector-virus-human host studies, we sequenced the
entire genome of one DENV-3 isolated from naturally infected Aedes aegypti from RJ in 2001 and characterized the
3’ UTR from strains isolated from mosquitoes and humans. Mosquitoes were pooled and submitted to virus
isolation in Ae. albopictus C6/36 cells and the infecting serotype was identified by immunofluorescence using
type-specific monoclonal antibody. Sequence analysis was performed using BioEdit software, the multiple
alignments were performed using CLUSTAL W and the phylogenetic analysis by MEGA 5, using the
Neighbor-joining method. Secondary structure prediction was performed by using the MFOLD program.
Results: Exclusive substitutions and a substitution leading to a stop codon on the NS5 gene were observed in the
DENV-3 isolated from a naturally infected Ae. aegypti and fully sequenced. As an 8- nucleotides deletion was
observed within the 11- nucleotides (nts) insertion on the variable region (VR) from the 3′UTR in this isolate, we
further sequenced other DENV-3 from both mosquitoes and humans. The majority of DENV-3 from RJ analyzed
were characterized by the 11-nts insertion in the VR of the 3′UTR, despite the observation of strains carrying the
8-nts deletion. The latter presented similar secondary structures, however not all strains presenting the 11-nts
insertion were similar in the predicted secondary structure.
Conclusions: The phylogeny based on the analysis of the complete genome and 3′UTR characterized the DENV-3
isolated from both vector and human host as belonging to Genotype III (GIII), despite the differences observed on
the 3’ UTR. Further studies are needed to address the role of those mutations in the transmission of the different
viral populations and vector competence. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue virus type 3 | pt_BR |
| Subject | Aedes aegypti | pt_BR |
| Subject | 3′UTR | pt_BR |
| DeCS | Dengue | pt_BR |
| DeCS | Vírus da Dengue | pt_BR |
| DeCS | Aedes | pt_BR |
