| Author | Bargut, Thereza Cristina Lonzetti | |
| Author | Ferreira, Tatiana Paula Teixeira | |
| Author | Daleprane, Julio Beltrame | |
| Author | Martins, Marco Aurélio | |
| Author | Silva, Patrícia Machado Rodrigues | |
| Author | Aguilla, Marcia Barbosa | |
| Access date | 2015-09-21T17:25:37Z | |
| Available date | 2015-09-21T17:25:37Z | |
| Document date | 2013 | |
| Citation | BARGUT, Thereza Cristina Lonzetti; et al. Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice. Plos One, v.8, n.9, 10p, Sept. 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11748 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Title | Fish Oil Has Beneficial Effects on Allergen-Induced Airway Inflammation and Hyperreactivity in Mice | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0075059 | pt_BR |
| Abstract | Background: Fish oil (FO) is rich in n-3 polyunsaturated fatty acids (PUFA), which have been suggested to be antiinflammatory
and are associated with improvement of several inflammatory diseases. In this study, we investigated
the influence of FO on allergen-induced lung inflammation and airway hyperreactivity in mice.
Methods: Male A/J mice were fed either a standard-chow (SC) or a FO diet (FO) for 8 weeks. After 4 weeks, each
group was further randomized for ovalbumin (SC-OVA and FO-OVA) or saline (SC-SAL and FO-SAL) challenge.
Resistance and elastance were measured at baseline and after aerosolized methacholine, 24h after the last
challenge. Bronchoalveolar lavage (BAL) was performed for leukocyte counts. Lung tissue mucus deposition,
peribronchiolar matrix deposition and eosinophil infiltration were quantified. Serum immunoglobulin E (IgE) and IgG1
(ref 2.2), lung IL-4, IL-5, IL-10, IL-13, IL-17, INFγ and eotaxin-1 and 2 were detected by ELISA and nuclear factor
kappa B (NFκB), GATA-3 and peroxisome proliferator-activated receptor gamma (PPARγ) expression was measured
by Western blot.
Results: Levels of serum IgE and IgG1 were significantly higher in OVA sensitized mice. OVA challenge resulted in
increased eosinophil infiltration, increased inflammatory cytokine production, peribronchiolar matrix and mucus
deposition and airway hyperreactivity to aerosolized methacholine. Elevated lung NFκB and GATA-3 expression was
noted in OVA-challenged mice. These changes were attenuated in mice fed with FO diet. Higher PPARγ expression
was also detected in the lungs from the FO-fed groups.
Conclusion: Our results demonstrate that FO intake attenuated classical asthma features by suppressing the
systemic sensitization, thus providing evidence that FO might be a prophylactic alternative for asthma prevention. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Centro Biomédico. Laboratório de Morfometria, Metabolismo e Doença Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Allergen-Induced Airway Inflammation | pt_BR |
| Subject | Fish Oil | pt_BR |
| Subject | Hyperreactivity | pt_BR |
| Subject | Asthma | pt_BR |
| DeCS | Dessensibilização Imunológica | pt_BR |
| DeCS | Hiper-Reatividade | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Inflamação | pt_BR |
| DeCS | Asma | pt_BR |
| xmlui.metadata.dc.subject.ods | 14 Vida na água | |
