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AutorOliveira, Désio Aurélio Farias de
AutorAlmeida, Vinícius Cotta de
AutorVila-Verde, Déa Maria S.
AutorRiederer, Ingo
AutorMeis, Juliana de
AutorSavino, Wilson
Fecha de acceso2015-09-21T17:25:38Z
Fecha de disponibilización2015-09-21T17:25:38Z
Fecha de publicación2013
ReferenciaOLIVEIRA, Désio Aurélio Farias de; et al. Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection. Mem Inst Oswaldo Cruz, v.108, n.7, p.825-831, Nov. 2013.pt_BR
ISSN1678-8060pt_BR
ISSN0074-0276pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/11750
Idiomaengpt_BR
EditorFundação Oswaldo Cruzpt_BR
Derechos de autoropen access
TítuloFibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infectionpt_BR
Tipo del documentoArticle
DOI10.1590/0074-0276130071pt_BR
Resumen en InglésDeveloping thymocytes interact with thymic epithelial cells (TECs) through cell-cell interactions, TEC-derived secretory moieties and extracellular matrix (ECM)-mediated interactions. These physiological interactions are crucial for normal thymocyte differentiation, but can be disrupted in pathological situations. Indeed, there is severe thymic atrophy in animals acutely infected with Trypanosoma cruzi due to CD4+CD8+ thymocyte depletion secondary to caspase-mediated apoptosis, together with changes in ECM deposition and thymocyte migration. We studied an in vitro model of TEC infection by T. cruzi and found that infected TEC cultures show a reduced number of cells, which was likely associated with decreased proliferative capacity, but not with increased cell death, as demonstrated by bromodeoxyuridine and annexin-V labelling. The infected TEC cultures exhibited increased expression of fibronectin (FN), laminin (LM) and type IV collagen. Importantly, treatment with FN increased the relative number of infected cells, whereas treatment with anti-FN or anti-LM antibodies resulted in lower infection rates. Consistent with these data, we observed increased thymocyte adhesion to infected TEC cultures. Overall, these results suggest that ECM molecules, particularly FN, facilitate infection of the thymic epithelium and that the consequent enhancement of ECM expression might be associated with changes in TEC-thymocyte interactions.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.pt_BR
Palavras clave en InglêsTrypanosoma cruzipt_BR
Palavras clave en InglêsThymic epithelial cellspt_BR
Palavras clave en InglêsThymocytespt_BR
Palavras clave en InglêsFibronectinpt_BR
Palavras clave en InglêsLamininpt_BR
DeCSTrypanosoma cruzipt_BR
DeCSLamininapt_BR
DeCSFibronectinaspt_BR
DeCSTimócitospt_BR


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