| Author | Vicentini, Fernando | |
| Author | Denadai, Wilson | |
| Author | Gomes, Yohanna Mayelle | |
| Author | Rose, Tatiana L. | |
| Author | Ferreira, Mônica S. R. | |
| Author | Moulac-Vaidye, Beatrice Le | |
| Author | Pendu, Jacques Le | |
| Author | Leite, José Paulo Gagliardi | |
| Author | Miagostovich, Marize Pereira | |
| Author | Spano, Liliana Cruz | |
| Access date | 2015-10-02T12:45:07Z | |
| Available date | 2015-10-02T12:45:07Z | |
| Document date | 2013 | |
| Citation | VICENTINI, Fernando; et al. Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil. Plos One, v.8, n.7, e69348, 9p, 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/11872 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Quilombola | pt_BR |
| Subject in Portuguese | Espírito Santo | pt_BR |
| Title | Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0069348 | pt_BR |
| Abstract | Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection
has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and
to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of
black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola
Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September
2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse
transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with
primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2
and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of
non-diarrheic children (p,0.05, Fisher’s exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples,
respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%)
and GI.4 (6.3%). Children infected with NoV showed the A (n = 6), O (n = 6), and B (n = 2) HBGA phenotypes, and 13 of them
were classified as secretors (Se) and one as a non secretor (se). Mutations of Se40, 171,216,357,428,739,960 were found for the FUT2
gene and mutations of Le59, 202, 314 for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children
were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic
Quilombola children consistent with other studies. However, children under 12 months were seven times more affected
than those between 1 and 5 years old. GII.12 was as frequent as GII.4 and GI.1 and GI.4 were described for the first time in
Brazil. Owing to the small number of cases studied, no clear pattern of susceptibility and/or HBGA resistance could be inferred. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil / Universidade Federal do Espírito Santo. Programa de Pós-Graduação em Doenças Infecciosas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Departamento de Ciências da Saúde. São Mateus, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Departamento de Patologia. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institut National de la Santé et de la Recherche Médicale. Nantes, France / Centre de Recherche en Cancérologie.Nantes, France / Université de Nantes. Nantes, France. | pt_BR |
| Affilliation | Institut National de la Santé et de la Recherche Médicale. Nantes, France / Centre de Recherche en Cancérologie.Nantes, France / Université de Nantes. Nantes, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Departamento de Patologia. Vitória, ES, Brasil. | pt_BR |
| Subject | Noroviruses (NoV) | pt_BR |
| Subject | Quilombola Children | pt_BR |
| Subject | Children | pt_BR |
| DeCS | Norovirus | pt_BR |
| DeCS | Crianças | pt_BR |
