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AutorTomich, Renata Graça Pinto
AutorBomfim, Maria Rosa Quaresma
AutorKoury, Matilde Cota
AutorPellegrini, Aiesca Oliveira
AutorPellegrin, Luiz Alberto
AutorKo, Albert Icksang
AutorStancioli, Edel Figueiredo Barbosa
Fecha de acceso2015-10-19T14:32:13Z
Fecha de disponibilización2015-10-19T14:32:13Z
Fecha de publicación2007
ReferenciaTOMICH, R. G. P. et al. Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test. Brazilian Journal of Microbiology, v. 38, p. 674-680, 2007.pt_BR
ISSN1517-8382
URIhttps://www.arca.fiocruz.br/handle/icict/11987
Idiomaengpt_BR
EditorSociedade Brasileira de Microbiologiapt_BR
Derechos de autoropen accesspt_BR
TítuloLeptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination testpt_BR
Tipo del documentoArticlept_BR
Resumen en InglésThis investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region’s climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major activity compatible of the conservation of natural resource of the region. Out of 282 samples of cattle serum analyzed, 143 (50.71%) were positive in MAT. The serovar Hardjo (genotypic Hardjoprajitno and Hardjobovis), Wolffi and Ballum showed the largest frequency of reactive samples. In the IgG ELISA rLipL32, 161 samples (57.09%) were positive. This result was higher than obtained by MAT (p<0.001). The sensitivity of the ELISA test was 99.30% and the specificity was 86.33%, based on the MAT. This test was shown to be a more sensitive, specific and accurate test for the diagnosis of bovine leptospirosis compared to the MAT.pt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil / Empresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, Brasilpt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasilpt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasilpt_BR
AfiliaciónEmpresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, Brasilpt_BR
AfiliaciónEmpresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, USApt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasilpt_BR
Palavras clave en InglêsBovine Leptospirosis,pt_BR
Palavras clave en InglêsIgG ELISA rLipL32pt_BR
Palavras clave en InglêsMATpt_BR


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