| Author | Ganime, A. C. | |
| Author | Leite, J. P. G. | |
| Author | Corrêa, A. de Abreu | |
| Author | Melgaço, F. G. | |
| Author | Costa, F. A. Carvalho | |
| Author | Miagostovich, M. P. | |
| Access date | 2015-10-20T12:21:41Z | |
| Available date | 2016-01-01T06:30:05Z | |
| Document date | 2015 | |
| ISSN | 0166-0934 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11997 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | Evaluation of the swab sampling method to recover viruses fromfomites | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.jviromet.2015.02.009 | |
| Abstract | tThe monitoring of virus contamination on fomites, especially at hospitals has been used for a more effec-tive evaluation of the microbiological quality of surfaces. Swab sampling is the method used currently,although the use of an internal control process (ICP) has not yet been assessed. The aim of this study is todetermine the recovery rate of murine norovirus 1 (MNV-1) and bacteriophage PP7 on different surfacesin order to assess their potential use as an ICP. For this purpose both viruses were spiked experimen-tally both on porous and non-porous formic as well as on rubberized surfaces. Quantitative PCR (qPCR)showed a variable efficiency with a percentage recovery ranging from 0.6 to 77% according to viruses andsurfaces. A global analysis suggested that MNV-1 could be used as a potential ICP for the swab samplingmethod. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbioologia e Parasitologia. Laboratório de Virologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Fomites | pt_BR |
| Subject | Swab | pt_BR |
| Subject | MNV-1 | pt_BR |
| Subject | Bacteriophage PP7 | pt_BR |
| Subject | Internal Control Process | pt_BR |
| DeCS | Fômites | pt_BR |
| DeCS | Bacteriófago PP7 | pt_BR |
