Author | Schachter, Julieta | |
Author | Delgado, Kelly Valcárcel | |
Author | Souza, Victor Barreto de | |
Author | Bou-Habib, Dumith Chequer | |
Author | Persechini, Pedro Muanis | |
Author | Fernandes, José Roberto Meyer | |
Access date | 2015-10-27T14:12:21Z | |
Available date | 2016-05-01T07:30:06Z | |
Document date | 2015 | |
Citation | SCHACHTER, Julieta; et al. nhibition of ecto-ATPase activities impairs HIV-1 infection of macrophages. Immunibiology, v.220, n.5, p.589-596, May 2015. | pt_BR |
ISSN | 0171-2985 | |
URI | https://www.arca.fiocruz.br/handle/icict/12094 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Inhibition of ecto-ATPase activities impairs HIV-1 infection of macrophages | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.imbio.2014.12.004 | |
Abstract | tNucleotides and nucleosides are secreted into extracellular media at different concentrations as a conse-quence of different physiologic and pathological conditions. Ecto-nucleotidases, enzymes present on thesurface of most cells, hydrolyze these extracellular nucleotides and reduce the concentration of them,thus affecting the activation of different nucleotide and nucleoside receptors. Also, ecto-nucleotidasesare present in a number of microorganisms and play important roles in host–pathogen interactions.Here, we characterized the ecto-ATPase activities present on the surface of HIV-1 particle and humanmacrophages as well. We found that the kinetic properties of HIV-1 and macrophage ecto-ATPases aresimilar, suggesting that the enzyme is the same. This ecto-ATPase activity was increased in macrophagesinfected in vitro with HIV-1. Using three different non-related ecto-ATPase inhibitors—POM-1, ARL67156and BG0—we showed that the inhibition of these macrophage and viral ecto-ATPase activities impairsHIV-1 infection. In addition, we also found that elevated extracellular concentrations of ATP inhibit HIV-1production by infected macrophages. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Campus de Xerém. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm). Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm). Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm). Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Ecto-ATPase | pt_BR |
Subject | CD39 | pt_BR |
Subject | Infected macrophages | pt_BR |
Subject | HIV Infection | pt_BR |
DeCS | Infecções por HIV | pt_BR |
DeCS | Macrófagos | pt_BR |
DeCS | Nucleotídeos | pt_BR |
DeCS | Nucleosídeos | pt_BR |
Embargo date | 2016-04-30 | |