Author | Matsunaga, James | |
Author | Medeiros, Marco Alberto | |
Author | Sanchez, Yolanda | |
Author | Werneid, Kristian F | |
Author | Ko, Albert Icksang | |
Access date | 2015-11-06T12:54:49Z | |
Available date | 2015-11-06T12:54:49Z | |
Document date | 2007 | |
Citation | MATSUNAGA, J. et al. Osmotic regulation of expression of two extracellular matrix-binding proteins and a haemolysin of Leptospira interrogans: differential effects on LigA and Sph2 extracellular release. Microbiology, v. 153, p. 3390–3398, 2007. | pt_BR |
ISSN | 1350-0872 | |
URI | https://www.arca.fiocruz.br/handle/icict/12131 | |
Language | eng | pt_BR |
Publisher | Microbiology Society | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leptospira | pt_BR |
Subject in Portuguese | Leptospira interrogans | pt_BR |
Subject in Portuguese | Leptospirose | pt_BR |
Title | Osmotic regulation of expression of two extracellular matrix-binding proteins and a haemolysin of Leptospira interrogans: differential effects on LigA and Sph2 extracellular release | pt_BR |
Type | Article | pt_BR |
DOI | 10.1099/mic.0.2007/007948-0 | |
Abstract | The life cycle of the pathogen Leptospira interrogans involves stages outside and inside the host.
Entry of L. interrogans from moist environments into the host is likely to be accompanied by the
induction of genes encoding virulence determinants and the concomitant repression of genes
encoding products required for survival outside of the host. The expression of the adhesin LigA,
the haemolysin Sph2 (Lk73.5) and the outer-membrane lipoprotein LipL36 of pathogenic
Leptospira species have been reported to be regulated by mammalian host signals. A previous
study demonstrated that raising the osmolarity of the leptospiral growth medium to physiological
levels encountered in the host by addition of various salts enhanced the levels of cell-associated
LigA and LigB and extracellular LigA. In this study, we systematically examined the effects of
osmotic upshift with ionic and non-ionic solutes on expression of the known mammalian
host-regulated leptospiral genes. The levels of cell-associated LigA, LigB and Sph2 increased at
physiological osmolarity, whereas LipL36 levels decreased, corresponding to changes in specific
transcript levels. These changes in expression occurred irrespective of whether sodium
chloride or sucrose was used as the solute. The increase of cellular LigA, LigB and Sph2 protein
levels occurred within hours of adding sodium chloride. Extracellular Sph2 levels increased when
either sodium chloride or sucrose was added to achieve physiological osmolarity. In contrast,
enhanced levels of extracellular LigA were observed only with an increase in ionic strength. These
results indicate that the mechanisms for release of LigA and Sph2 differ during host infection.
Thus, osmolarity not only affects leptospiral gene expression by affecting transcript levels of
putative virulence determinants but also affects the release of such proteins into the surroundings. | pt_BR |
Affilliation | VA Greater Los Angeles Healthcare System. Research Service. Los Angeles, CA, USA / UCLA David Geffen School of Medicine. Los Angeles, CA, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | VA Greater Los Angeles Healthcare System. Research Service. Los Angeles, CA, USA | pt_BR |
Affilliation | VA Greater Los Angeles Healthcare System. Research Service. Los Angeles, CA, USA | pt_BR |
Affilliation | Weill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, NY, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |