| Author | Silva, Amaro E. Trindade | |
| Author | Rua, Cintia P. J. | |
| Author | Andrade, Bruno G. N. | |
| Author | Vicente, Ana Carolina Paulo | |
| Author | Silva, Genivaldo G. Z. | |
| Author | Berlinck, Roberto G. S. | |
| Author | Thompson, Fabiano L. | |
| Access date | 2016-01-19T11:00:12Z | |
| Available date | 2016-01-19T11:00:12Z | |
| Document date | 2013 | |
| Citation | SILVA, Amaro E. Trindade; et al. Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean. Applied and Environmental Microbiology, v.79, n.5, p.1598-1605, Mar. 2013. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/12551 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Title | Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean | pt_BR |
| Type | Article | |
| DOI | 10.1128/AEM.03354-12 | |
| Abstract | Microbes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The
synthesis of such secondary metabolites involves type I polyketide synthases (PKSs), which are enzymes that reach a maximum
complexity degree in bacteria. The Haplosclerida sponge Arenosclera brasiliensis hosts a complex microbiota and is the source of
arenosclerins, alkaloids with cytotoxic and antibacterial activity. In the present investigation, we performed high-throughput
sequencing of the ketosynthase (KS) amplicon to investigate the diversity of PKS genes present in the metagenome of A. brasiliensis.
Almost 4,000 ketosynthase reads were recovered, with about 90% annotated automatically as bacterial. A total of 235 bacterial
KS contigs was rigorously assembled from this sequence pool and submitted to phylogenetic analysis. A great diversity of
six type I PKS groups has been consistently detected in our phylogenetic reconstructions, including a novel and A. brasiliensisexclusive
group. Our study is the first to reveal the diversity of type I PKS genes in A. brasiliensis as well as the potential of its
microbiome to serve as a source of new polyketides. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química de São Carlos. São Carlos, SP, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | San Diego State University. Department of Computer Science. San Diego, California, USA. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química de São Carlos. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Polyketide Synthase | pt_BR |
| Subject | Gene Diversity | pt_BR |
| Subject | Sponge | pt_BR |
| Subject | Arenosclera brasiliensis | pt_BR |
| DeCS | Microbiota | pt_BR |
| DeCS | Policetídeo Sintases | pt_BR |
| e-ISSN | 1098-5336 | |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
