| Author | Morais, Lena Lilian Canto de Sá | |
| Author | Garza, Daniel Rios | |
| Author | Loureiro, Edvaldo Carlos Brito | |
| Author | Vale, Elivam Rodrigues | |
| Author | Santos, Denise Suéllem Amorim de Sousa | |
| Author | Corrêa, Vanessa Cavaleiro | |
| Author | Sousa, Nayara Rufino | |
| Author | Gurjão, Tereza Cristina Monteiro | |
| Author | Santos, Elisabeth Conceição de Oliveira | |
| Author | Vieira, Verônica Viana | |
| Author | Fonseca, Erica Lourenço da | |
| Author | Vicente, Ana Carolina Paulo | |
| Access date | 2016-01-19T11:54:28Z | |
| Available date | 2016-01-19T11:54:28Z | |
| Document date | 2013 | |
| Citation | MORAIS, Lena Lillian Canto de Sá; et al. Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region. Plos One, v.8, n.11, e81372, 12p, 2013. | pt_BR |
| ISSN | 10.1371/journal.pone.0081372 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12552 | |
| Language | eng | pt_BR |
| Publisher | Plos One | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Amazônia | pt_BR |
| Subject in Portuguese | Estudos genéticos | pt_BR |
| Subject in Portuguese | Estudos populacionais | pt_BR |
| Title | Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region | pt_BR |
| Type | Article | |
| Abstract | Vibrio cholerae is a natural inhabitant of many aquatic environments in the world. Biotypes harboring similar
virulence-related gene clusters are the causative agents of epidemic cholera, but the majority of strains are harmless
to humans. Since 1971, environmental surveillance for potentially pathogenic V. cholerae has resulted in the isolation
of many strains from the Brazilian Amazon aquatic ecosystem. Most of these strains are from the non-O1/non-O139
serogroups (NAGs), but toxigenic O1 strains were isolated during the Latin America cholera epidemic in the region
(1991-1996). A collection of environmental V. cholerae strains from the Brazilian Amazon belonging to pre-epidemic
(1977-1990), epidemic (1991-1996), and post-epidemic (1996-2007) periods in the region, was analyzed. The
presence of genes related to virulence within the species and the genetic relationship among the strains were
studied. These variables and the information available concerning the strains were used to build a Bayesian
multivariate dependency model to distinguish the importance of each variable in determining the others. Some genes
related to the epidemic strains were found in environmental NAGs during and after the epidemic. Significant diversity
among the virulence-related gene content was observed among O1 strains isolated from the environment during the
epidemic period, but not from clinical isolates, which were analyzed as controls. Despite this diversity, these strains
exhibited similar PFGE profiles. PFGE profiles were significant while separating potentially epidemic clones from
indigenous strains. No significant correlation with isolation source, place or period was observed. The presence of
the WASA-1 prophage significantly correlated with serogroups, PFGE profiles, and the presence of virulence-related
genes. This study provides a broad characterization of the environmental V. cholerae population from the Amazon,
and also highlights the importance of identifying precisely defined genetic markers such as the WASA-1 prophage for
the surveillance of cholera. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Bacteriologia. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Vibrio Cholorae | pt_BR |
| Subject | Brazilian Amazon aquatic ecosystem | pt_BR |
| Subject | Environmental V. cholerae | pt_BR |
| Subject | Genetic study | pt_BR |
| DeCS | Vibrio Cholorae | pt_BR |
| DeCS | Cólera | pt_BR |
| e-ISSN | 1932-6203 | |
