| Author | Americo, Juliana Alves | |
| Author | Dondero, Francesco | |
| Author | Moraes, Milton Ozório | |
| Author | Allodi, Silvana | |
| Author | Rebelo, Mauro de Freitas | |
| Access date | 2016-01-19T14:31:06Z | |
| Available date | 2016-01-19T14:31:06Z | |
| Document date | 2013 | |
| Citation | AMERICO, Juliana Alves et al. Gene discovery in the tropical scallop Nodipecten nodosus: construction and sequencing of a normalized cDNA library. Marine Environmental Research, v. 91, p. 34-40, Oct. 2013. | |
| ISSN | 0141-1136 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12556 | |
| Description | Produção científica do Laboratório de Hanseníase. | pt_BR |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | open access | |
| Title | Gene discovery in the tropical scallop Nodipecten nodosus: construction and sequencing of a normalized cDNA library | en_US |
| Type | Article | |
| DOI | 10.1016/j.marenvres.2013.03.008 | |
| Abstract | We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0 × 10⁶ cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Università del Piemonte Orientale Amedeo Avogadro. Department of Science and Technological Innovation. Alessandria, Italy. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Subject | EST | en_US |
| Subject | mRNA | en_US |
| Subject | Lion’s Paw scallop | en_US |
| Subject | Aquaculture | en_US |
| Subject | Stress | en_US |
| DeCS | RNA Mensageiro | pt_BR |
| DeCS | Estresse | pt_BR |
| DeCS | Biblioteca Gênica | pt_BR |
| e-ISSN | 1879-0291 | |
