| Author | Krawczak, Felipe da Silva | |
| Author | Reis, Ilka Afonso | |
| Author | Silveira, Julia Angélica da | |
| Author | Avelar, Daniel Moreira | |
| Author | Marcelino, Andreza Pain | |
| Author | Werneck, Guilherme Loureiro | |
| Author | Labruna, Marcelo Bahia | |
| Author | Paz, Gustavo Fontes | |
| Access date | 2016-01-25T17:21:46Z | |
| Available date | 2016-01-25T17:21:46Z | |
| Document date | 2015 | |
| Citation | KRAWCZAK, Felipe da Silva et al. Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? Rev. Soc. Bras. Med. Trop., vol.48 n.1, p. 64-68, 2015 | pt_BR |
| ISSN | 0037-8682 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12607 | |
| Language | eng | pt_BR |
| Publisher | Sociedade Brasileira de Medicina Tropical | pt_BR |
| Rights | open access | pt_BR |
| Title | Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0037-8682-0291-2014 | |
| Abstract | INTRODUCTION: The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites.
METHODS: The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios).
RESULTS: Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05).
CONCLUSIONS: In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react. | pt_BR |
| Affilliation | Universidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Estatística. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Departamento de Epidemiologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG Brasil. | pt_BR |
| Subject | Immunochromatographic test | pt_BR |
| Subject | Enzyme-linked immunosorbent assay | pt_BR |
| Subject | Immunofluorescence antibody test | pt_BR |
| Subject | Canine visceral leishmaniasis | pt_BR |
