| Author | Gontijo, Raquel Carvalho | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Silva, Matheus Fernandes Costa e | |
| Author | Martins Filho, Olindo Assis | |
| Author | Quaresma, Patrícia Flávia | |
| Author | Freire, Janaira de Moura | |
| Author | Moreno, Elizabeth de Castro | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Gontijo, Celia Maria Ferreira | |
| Access date | 2016-01-26T13:26:14Z | |
| Available date | 2016-01-26T13:26:14Z | |
| Document date | 2015 | |
| Citation | GONTIJO, Raquel Carvalho et al. Protective profile involving CD23/IgE-mediated NO release is a hallmark of cutaneous leishmaniasis patients from the Xakriabá indigenous community in Minas Gerais, Brazil. Scandinavian Journal of Immunology, vol. 81, p. 515–524, 2015 | pt_BR |
| ISSN | 0300-9475 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12618 | |
| Language | eng | pt_BR |
| Publisher | John Wiley & Sons Ltd. | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Protective profile involving CD23/IgE-mediated NO release is a hallmark of cutaneous leishmaniasis patients from the Xakriabá indigenous community in Minas Gerais, Brazil. | pt_BR |
| Type | Article | |
| DOI | 10.1111/sji.12294 | |
| Abstract | In this study, we described, for the first time, specific aspects of an anti-Leishmania immune response in a Brazilian Xakriabá indigenous community. Induction of an intracellular NO pathway, triggered by the binding of IgE to CD23 receptor in IFN-γ/IL-4 cytokines environment, was evaluated in localized cutaneous leishmaniasis (LCL) carriers and positive Montenegro skin test (MST) individuals without skin lesion (MT(+) SL(-)). Our data demonstrated that the higher frequency of CD23(+) CD14(+) monocytes and the increased serum levels of IgE observed in the LCL group were even higher in LCL carriers with late lesions (LCL≥60). Furthermore, patients with LCL presented increased NO production after Leishmania (Viannia) braziliensis stimulation and this NO profile was independent of the time of the lesion (recent LCL<60 or late LCL≥60). We also showed that the increased frequency of IFN-γ(+) and IL-4(+) CD4(+) T cells is related to the MT(+) SL(-) group. The results of biomarker signature curves demonstrated that in the MT(+) SL(-) group, the index signature was characterized by DAF-2T(+) CD14(+)/IL-4(+) CD8(+)/IFN-γ(+) CD4(+)/IL-4(+) CD4(+). On the other hand, the LCL group presented a higher index of DAF-2T(+) CD14(+)/CD23(+) CD14(+)/IL-4(+) CD8(+), associated with a lower index of IFN-γ(+) CD8(+). Considering the time of lesion, data analysis demonstrated that the main differences observed were highlighted in LCL<60 patients, with a higher index of CD23(+) CD14(+), which was also present in LCL≥60 patients. In conclusion, our data suggest that the protective immune response involving CD23-IgE-mediated NO release is a hallmark of patients with LCL. However, in MT(+) SL(-) individuals, another different leishmanicidal mechanism seems to be involved. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Biomarcadores para Diagnostico e Monitoramento. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Leishmaniasis, Cutaneous/immunology | pt_BR |
| Subject | Skin Tests/methods | pt_BR |
| Subject | Antigens, CD14/blood | pt_BR |
| Subject | Immunoglobulin E/blood | pt_BR |
| Subject | Interferon-gamma/immunology | pt_BR |
