Author | Macedo, Sharon Rose Aragão | |
Author | Nicolete, Larissa Deadame de Figueiredo | |
Author | Ferreira, Amália dos Santos | |
Author | Barros, Neuza Biguinati de | |
Author | Nicolete, Roberto | |
Access date | 2016-01-26T14:37:54Z | |
Document date | 2015 | |
Citation | NICOLETE, R. et al. The pentavalent antimonial therapy against experimental Leishmania amazonensis infection is more effective under the inhibition of the NF-κB pathway. International Immunopharmacology (Print), v. 28, p. 554-559, 2015. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/12621 | pt_BR |
Language | eng | pt_BR |
Publisher | ELSEVIER | pt_BR |
Rights | open access | pt_BR |
Title | The pentavalent antimonial therapy against experimental Leishmania amazonensis infection is more effective under the inhibition of the NF-kB pathway | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.intimp.2015.07.020 | |
Abstract | During Leishmania infection, host immune response is important to prevent the growth/survival of intracellular
amastigotes. In this study, we evaluated in vitro and in vivo whether or not during Leishmania amazonensis infection, pentavalent antimonial treatment/therapy could be more effective under TNF-α inhibition. Both L. amazonensis-infected macrophages (in vitro model) and mice (in vivo model) were treated with a nuclear
factor-kB (NF-kB) inhibitor and with Glucantime®, alone and in combined administrations. The in vitro amastigote counts, cytokines and nitrites' production were assessed after 48 h incubation with the drugs. Paw lesion sizes and amastigote counts were also evaluated in vivo. Quantification of IL-1β from the infected tissue was performed. In vitro results show that when infected macrophages were incubated with QNZ + Glucantime®, a greater clearance was observed for the amastigotes' growth and this was related to greater nitrite production compared to the group that was only infected. In vivo results show that mice that received the combined treatment had their paw lesion sizes and amastigote nests inside the macrophages greatly diminished, correlating with increased IL-1β levels. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biotecnologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biotecnologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biotecnologia Aplicada à Saúde. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biotecnologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Laboratório de Biotecnologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brazil. / Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal. Porto Velho, RO, Brazil. | pt_BR |
Subject | Leishmania amazonensis | pt_BR |
Subject | Antimonial therapy | pt_BR |
Subject | TNF-α inhibition | pt_BR |
Subject | IL-1β | pt_BR |
Subject | Nitric oxide | pt_BR |
Subject | Immune response | pt_BR |
Embargo date | 2016-11-30 | |