| Author | Soprani, Taisla | |
| Author | Uliana, Vinicius Kuffer | |
| Author | Ribeiro Jr, Rogerio Faustino | |
| Author | Lisboa Jr, Sergio | |
| Author | Maretto, Gabriella Xavier | |
| Author | Ferreira, André Teixeira da Silva | |
| Author | Perales, Jonas | |
| Author | Stefanon, Ivanita | |
| Author | Figueiredo, Suely Gomes de | |
| Access date | 2016-03-08T14:25:34Z | |
| Available date | 2016-03-08T14:25:34Z | |
| Document date | 2015 | |
| Citation | SOPRANI, Taisla; et al. Cardiac protein changes in rats after soybean oil treatment: a proteomic study. Lipids in Health and Disease, v.14:26, 9p, 2015. | pt_BR |
| ISSN | 1476-511X | |
| URI | https://www.arca.fiocruz.br/handle/icict/13051 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Proteínas do coração | pt_BR |
| Subject in Portuguese | Óleo de soja | pt_BR |
| Subject in Portuguese | Proteômica comparativa | pt_BR |
| Subject in Portuguese | Ácidos graxos poliinsaturados | pt_BR |
| Subject in Portuguese | Electroforese em gel bidimensional | pt_BR |
| Title | Cardiac protein changes in rats after soybean oil treatment: a proteomic study | pt_BR |
| Type | Article | |
| DOI | 10.1186/s12944-015-0024-3. | |
| Abstract | Background: Several studies show that the consumption of vegetable oils, such as soybean oil, rich in
polyunsaturated fatty acids (PUFAs) has beneficial health effects by preventing or reducing the risk factors of
cardiovascular diseases. While the demonstration of beneficial effects of the consumption of unsaturated fatty acids
on the cardiovascular system has been proven in a macroscopic level, the molecular/cellular mechanisms
responsible for this phenomenon are poorly understood.
Methods: In this work, a comparative proteomic approach, two-dimensional gel electrophoresis (2-DE) coupled to mass
spectrometry (MALDI-TOF/TOF), was applied to investigate proteome differences in the left ventricle (LV) of rats that
received 0.1 mL of soybean oil intramuscularly for 15 days (treated group - TR) and rats that had not (control group - CT).
Results: Soybean oil treatment improved left ventricular function, TR animals presented lower value of LVEDP and
significantly changed LV proteome. The protein profile of VE revealed differences in the expression of 60 protein spots
(p < 0.05) between the experimental groups (CT and TR), 14 of those were identified by MS and MS/MS, and 12 of the 14
being non-redundant proteins. Robust changes were detected in proteins involved in cellular structure and antioxidant
system and muscular contraction.
Conclusions: The TR group presented an increase in the intensity of proteins involved in muscle contraction (myosin
light chain-3 (3-MCL), creatine kinase M (CKM)) and thireodoxin, an antioxidant enzyme. Low intensity cytoskeletal protein,
desmin, was also detected in TR animals. The results suggest that soybean oil induces changes in the levels of heart
proteins which may partially account for the underlying mechanisms involved in the benefits provided by oils rich in
polyunsaturated fatty acids. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo(UFES). Pós-Graduação em Ciências Fisiológicas. Vitória, ES, Brasil. | pt_BR |
| Subject | Comparative proteomic | pt_BR |
| Subject | Soybean oil | pt_BR |
| Subject | MS | pt_BR |
| Subject | Heart proteins | pt_BR |
