Author | Pereira, Polyana Silva | |
Author | Borghi, Miria | |
Author | Araújo, Carlos Felipe Machado de | |
Author | Aires, Caio Augusto Martins | |
Author | Oliveira, Jane Cleide Ribeiro | |
Author | Asensi, Marise Dutra | |
Author | Assef, Ana Paula D’Alincourt Carvalho | |
Access date | 2016-03-10T14:33:42Z | |
Available date | 2016-03-10T14:33:42Z | |
Document date | 2015 | |
Citation | PEREIRA, Polyana Silva; etal. Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil. Antimicrobial Agents and Chemotherapy, v.59, n.8, p.4453-4456, Aug. 2015. | pt_BR |
ISSN | 0066-4804 | |
URI | https://www.arca.fiocruz.br/handle/icict/13097 | |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | |
Title | Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil | pt_BR |
Type | Article | |
DOI | 10.1128/AAC.04243-14 | |
Abstract | Enzymes of the OXA-48 family have become some of the most important beta-lactamases in the world. A new OXA-48 variant
(OXA-370) was first described for an Enterobacter hormaechei strain isolated in Rio Grande do Sul (southern region of Brazil) in
2013. Here we report detection of the blaOXA-370 gene in 24 isolates belonging to three Enterobacteriaceae species (22 Klebsiella
pneumoniae isolates, 1 Enterobacter cloacae isolate, and 1 Enterobacter aerogenes isolate) collected from five hospitals in Rio de
Janeiro, Brazil, in 2013 and 2014. The isolates showed a multidrug resistance profile, and 12.5% were resistant to polymyxin B.
Besides blaOXA-370, no other carbapenemase genes were observed by PCR, whereas blaOXA-1 was found in all isolates and 22 isolates
(91.6%) possessed blaCTX-M-15. Molecular typing of the K. pneumoniae isolates by pulsed-field gel electrophoresis (PFGE)
showed the presence of two clonal groups, i.e., KpA (21 isolates) and KpB (1 isolate). KpA was characterized as sequence type 16
(ST16) and KpB as ST1041 by multilocus sequence typing (MLST). ST16 has been observed for KPC-producing K. pneumoniae
in Rio de Janeiro. Plasmid analysis performed with six representative OXA-370-producing isolates showed plasmids harboring
the blaOXA-370 gene in all strains, ranging from 25 kb to 150 kb. This study suggests that there is an urgent need to investigate the
presence of OXA-370 and dissemination of the K. pneumoniae ST16 clone carrying this gene in Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Laboratório Central de Saúde Pública do Rio de Janeiro Noel Nutels. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Clonal Dissemination | pt_BR |
Subject | OXA-370-Producing | pt_BR |
Subject | Klebsiella pneumoniae | pt_BR |
Subject | Rio de Janeiro | pt_BR |
Subject | Enterobacteriaceae | pt_BR |
DeCS | Klebsiella pneumoniae | pt_BR |
DeCS | beta-Lactamases | pt_BR |
DeCS | Infecções por Enterobacteriaceae | pt_BR |
e-ISSN | 1098-6596 | |