| Author | Güldner, Andreas | |
| Author | Maron-Gutierrez, Tatiana | |
| Author | Abreu, Soraia Carvalho | |
| Author | Xisto, Debora Gonçalves | |
| Author | Senegaglia, Alexandra Cristina | |
| Author | Barcelos, Patty Rose da Silva | |
| Author | Silva, Johnatas Dutra | |
| Author | Brofman, Paulo | |
| Author | Abreu, Marcelo Gama de | |
| Author | Rocco, Patricia Rieken Macedo | |
| Access date | 2016-03-22T17:10:21Z | |
| Available date | 2016-03-22T17:10:21Z | |
| Document date | 2015 | |
| Citation | GÜLDNER, Andreas; et al. Expanded endothelial progenitor cells mitigate lung injury in septic mice. Stem Cell Research & Therapy, v.6:230, 8p, 2015. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/13246 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Terapias celulares | pt_BR |
| Title | Expanded endothelial progenitor cells mitigate lung injury in septic mice | pt_BR |
| Type | Article | |
| DOI | 10.1186/s13287-015-0226-7 | |
| Abstract | Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation and puncture-induced
sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has
compared the effects of non-expanded EPCs (EPC-NEXP) with those of expanded EPCs (EPC-EXP) and mesenchymal
stromal cells of human (MSC-HUMAN) and mouse (MSC-MICE) origin in experimental sepsis. One day after cecal
ligation and puncture sepsis induction, BALB/c mice were randomized to receive saline, EPC-EXP, EPC-NEXP,
MSC-HUMAN or MSC-MICE (1 × 105
) intravenously. EPC-EXP, EPC-NEXP, MSC-HUMAN, and MSC-MICE displayed
differences in phenotypic characterization. On days 1 and 3, cecal ligation and puncture mice showed decreased
survival rate, and increased elastance, diffuse alveolar damage, and levels of interleukin (IL)-1β, IL-6, IL-10, tumor
necrosis factor-α, vascular endothelial growth factor, and platelet-derived growth factor in lung tissue. EPC-EXP and
MSC-HUMAN had reduced elastance, diffuse alveolar damage, and platelet-derived growth factor compared to
no-cell treatment. Tumor necrosis factor-α levels decreased in the EPC-EXP, MSC-HUMAN, and MSC-MICE groups.
IL-1β levels decreased in the EPC-EXP group, while IL-10 decreased in the MSC-MICE. IL-6 levels decreased both in
the EPC-EXP and MSC-MICE groups. Vascular endothelial growth factor levels were reduced regardless of therapy. In
conclusion, EPC-EXP and MSC-HUMAN yielded better lung function and reduced histologic damage in septic mice. | pt_BR |
| Affilliation | Technische Universität Dresden. University Hospital Dresden. Department of Anesthesiology and Intensive Care Medicine. Dredesm Germany / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Technische Universität Dresden. University Hospital Dresden. Department of Anesthesiology and Intensive Care Medicine. Dredesm Germany. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Sepsis | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | Growth factors | pt_BR |
| Subject | Cell therapies | pt_BR |
| DeCS | Peptídeos e Proteínas de Sinalização Intercelular | pt_BR |
| DeCS | Inflamação | pt_BR |
| DeCS | Sepse | pt_BR |
| e-ISSN | 1757-6512 | |
